Document Type: Original Research Article

Authors

Department of Chemistry, College of Science, university of Agriculture, Makurdi Nigeria

10.33945/SAMI/PCBR.2019.2.7

Abstract

Study evaluates the in-vitro anti-microbial and anti-ulcer activity of Cassia sieberiana leaf extracts on Helicobacter pylori the major etiological agent of chronic active gastritis and peptic ulcer disease also called gastric carcinoma. Crude extracts of cassia sieberiana were screened for their antimicrobial and antiulcer activities. Antimicrobial assay and in-vitro anti-ulcer (H. pylori) was performed using Agar Diffusion Method and Broth Dilution Method. Ethyl acetate extract had the highest diameter of zones of inhibition (30 mm). Ethyl acetate and methanol extracts had the same Minimum inhibitory concentrations of 10 mg/mL against Streptococus feacalis, Escherichia coli, Helicobacter pylori, shigella dysenteriae and Candida stellafoidea but for Campylobacter jejuni which had a minimum inhibitory concentration at 20 mg/mL for methanol extract. Methanol extract had the best minimum bactericidal/fungicidal concentration (40 mg/mL) on the pathogens. The results showed that Cassia sieberiana exhibited strong antimicrobial activity against H. pylori at two different concentrations of 20, 40mg/mL.

Graphical Abstract

Keywords

Main Subjects

References

[1] F. Hamill, S. Apio, N. Mubiru, R. Bukenya-Ziraba, M. Mosango, O. Maganyi and D. Soejarto, Traditional herbal drugs of Southern Uganda, II: literature analysis and antimicrobial assays. Journal of Ethnopharmacology,  84 (2003)  57-78.

[2] Y. Dikko, M. Khan, T. Tor-Anyiin, J. Anyam and U. Linus, In vitro Antimicrobial Activity of Fruit Pulp Extracts of Azanza garckeana (F. Hoffm.) Exell & Hillc. and Isolation of One of its Active Principles, Betulinic Acid. Journal of Pharmaceutical Research International, (2016)  1-10.

[3] A. GOA, S. Chollom, J. Gotep, N. Gambo, A. Tyem, I. Okeke, O. Nwankiti and A. Okwori, Evaluation of antimicrobial potential of ethanolic leaf and stem bark extracts of Tamarindus indica. (2012) 

[4] S. Al-Daihan, M. Al-Faham, N. Al-shawi, R. Almayman, A. Brnawi and R. shafi Bhat, Antibacterial activity and phytochemical screening of some medicinal plants commonly used in Saudi Arabia against selected pathogenic microorganisms. Journal of King Saud University-Science,  25 (2013)  115-120.

[5] D.A. Awomukwu, B.L. Nyananyo, A.I. Ikpeama and C.U. Adieze, Comparative chemical constituents of some Cassia species and their pharmacognistic importance in South Eastern Nigeria. Science Journal of Chemistry,  3 (2015)  40-49.

[6] M. Arbonnier, Trees, shrubs and lianas of West African dry zones. (2004): Quae.

[7] A. Sofowora, Recent trends in research into African medicinal plants. Journal of ethnopharmacology,  38 (1993)  197-208.

[8] L. Buwa and J. Van Staden, Antibacterial and antifungal activity of traditional medicinal plants used against venereal diseases in South Africa. Journal of ethnopharmacology,  103 (2006)  139-142.

[9] A. Babaei and A.R. Taheri, Direct electrochemistry and electrocatalysis of myoglobin immobilized on a novel chitosan-nickel hydroxide nanoparticles-carbon nanotubes biocomposite modified glassy carbon electrode. Anal. Bioanal. Electrochem,  4 (2012)  342-356.

[10] A. Taheri and S. Mohammadi, Ultrasensitive and selective non-enzymatic glucose detection based on pt electrode modified by carbon nanotubes@ graphene oxide/nickel hydroxide-Nafion hybrid composite in alkaline media. Progress in Chemical and Biochemical Research,  1 (2018)  1-10.

[11] A. Michael, Trees, shrubs and lianas of West Africa dry zones. Cirad-Margraf Publishers Gmbh-Mnhn, pp573, (2004) 

[12] R. Keay, Trees of Nigeria (A revised edition of Nigeria Trees) Oxford University press. New York, USA, (1989)  318-323.

[13] S.E. Atawodi, M.L. Liman, O.D. Olowoniyi and G.O. Adejo, Some Wild Herbaceous Plants of Nigeria: A Biological, Pharmacognostic and Phytochemical Review, in Medicinal and Aromatic Plants of the World-Africa Volume 3. (2017), Springer.  249-282.

[14] T.L. Srinivas, S.M. Lakshmi, S.N. Shama, G.K. Reddy and K. Prasanna, Medicinal plants as anti-ulcer agents. Journal of Pharmacognosy and Phytochemistry,  2 (2013)  91-97.

[15] N. Dashputre and N. Naikwade, Evaluation of anti-ulcer activity of methanolic extract of Abutilon indicum Linn leaves in experimental rats. Int J Pharm Sci Drug Res,  3 (2011)  97-100.

[16] D.J.E. Cullen, G.M. Hawkey, D.C. Greenwood, H. Humphreys, V. Shepherd, R.F.A. Logan and C.J. Hawkey, Peptic ulcer bleeding in the elderly: relative roles ofHelicobacter pylori and non-steroidal anti-inflammatory drugs. . Gut,  41 (1997)  459-462.

[17] R. Jalilian and A. Taheri, Synthesis and application of a novel core-shell-shell magnetic ion imprinted polymer as a selective adsorbent of trace amounts of silver ions. e-Polymers,  18 (2018)  123-134.

[18] C. Kumari, C. Prasad and J.S. Ramulu, Determination of in-vitro and in-vivo activities of Aloe vera L. against H. pylori. International Journal of Pharma and Bio Sciences,  1 (2010) 

[19] S. Heron and E. Yarnell, A Botanical Protocol for Helicobacter pylori Infection. Alternative and Complementary Therapies,  6 (2000)  60-68.

[20] M. Nabati and V. Bodaghi-Namileh, Molecular Modeling of 3-(1,3-Dioxoisoindolin-2-yl)benzyl Nitrate and its Molecular Docking Study with Phosphodiesterase-5 (PDE5). Advanced Journal of Chemistry, Section A: Theoretical, Engineering and Applied Chemistry,  3 (2020)  58-69.

[21] H. Aliyan, Friedel-Crafts alkylation of indoles with epoxides using PW12-APTES@SBA-15. Iranian Chemical Communication,  6 (2018)  325-333.

[22] M. Nabati, E. Lohrasbi, H. Sabahnoo, V. Bodaghi-Namileh, M. Mazidi, H. Mohammadnejad-Mehrabani, A. Tavakkoli and A. Gervand, In Silico Study of Metoclopramide as A Small Molecule of Dopamine D2 Receptor: a Quantum-Mechanical (QM) Based Molecular Docking Treatment. Chemical Methodologies,  4 (2020)  19-33.

[23] I. Abdurrahman, H. Yu-Lai, Y. Cai-Xia and T.E. Hagr, Identification and characterization of diterpenes from ethyl acetate fraction of stem barks of Boswellia papyrifera (del) hochst, sudanese medicinal plant. Asian Journal of Green Chemistry,  3 (2019)  322-335.

[24] M. Thabrew and L. Arawwawala, An Overview of In Vivo and In Vitro Models that can be used for Evaluating Anti-Gastric Ulcer Potential of Medicinal Plants. (2018) 

[25] E. Sheen and G. Triadafilopoulos, Adverse effects of long-term proton pump inhibitor therapy. Digestive diseases and sciences,  56 (2011)  931-950.

[26] P. Mahendran, A. Vanisree and C. Shyamala Devi, The antiulcer activity of Garcinia cambogia extract against indomethacin‐induced gastric ulcer in rats. Phytotherapy research,  16 (2002)  80-83.

[27] M.K. Aadesariya, V.R. Ram and P.N. Dave, Investigation of phytochemicals in methanolic leaves extracts of Abutilon pannosum and Grewia tenax by Q-TOF LC/MS. Progress in Chemical and Biochemical Research,  2 (2019)  13-19.

[28] T. Tor-Anyiin, J. Igoli, J. Anyam and J. Anyam, ISOLATION AND ANTIMICROBIAL ACTIVITY OF SARRACENIN FROM ROOT BARK OF STRYCHNOS SPINOSA. Journal of Chemical Society of Nigeria,  40 (2015) 

[29] A. Smith-Palmer, J. Stewart and L. Fyfe, Antimicrobial properties of plant essential oils and essences against five important food-borne pathogens. Letters in applied microbiology,  26 (1998)  118-122.