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Document Type : Original Research Article


1 Department of Chemistry, Kosar University of Bojnord, Bojnord, Iran

2 Department of Biology, Payame Noor University, P.O. BOX 19395-4697 Tehran, Iran


In this research, the volatile compounds of Oleo gum resin of   Ferula gummosa were extracted from the protected area of Saluk located in North Khorasan province of Iran by water distillation method and the chemical compounds were identified by GC-MS and reported for the first time. In the studied plant essential oil, 42 compounds that made up 95.78% of essential oils have been identified, the main components of which are β-pinene (44.51%), α-pinene (11.49%), para-cymene (2.48%), 3-carene (5.74%), and 2-isopropyl 4-methylanisole (4.58%). In the following, five identified main compounds of this plant were compared with the main compounds of 26 ecotypes that were previously reported. By comparing the five main compounds of these 27 ecotypes, it was found that the main compounds of these samples are classified in the category of monoterpenes, sesquiterpenes, and esters, and also by drawing a cluster dendrogram using R software, the proximity and distance of these samples in terms of the type of main compounds, it is clustered into three clusters including samples with similar compounds. This study can be effective in continuing the research on eco-phytochemistry and ethnobotanical characteristics of Frula gummosa.

Graphical Abstract

Chemical profile of Ferula gummosa growing in the Saluk protected area of Iran and comparing the results with other habitats.


Main Subjects

  1. Mozaffarian V. A dictionary of Iranian plant names, Tehran: Farhang Moaser; 1996; 396:396-8.
  2. Mohammadhosseini M, Mahdavi B, Shahnama M. Chemical composition of essential oils from aerial parts of Ferula gummosa (Apiaceae) in Jajarm Region, Iran using traditional hydrodistillation and solvent-free microwave extraction methods: A comparative approach, Journal of Essential Oil Bearing Plants; 2015 Nov 2; 18(6):1321-8.
  3. Pavela R, Morshedloo MR, Lupidi G, Carolla G, Barboni L, Quassinti L, Bramucci M, Vitali LA, Petrelli D, Kavallieratos NG, Boukouvala MC. The volatile oils from the oleo-gum-resins of Ferula assa-foetida and Ferula gummosa: a comprehensive investigation of their insecticidal activity and eco-toxicological effects, Food and Chemical Toxicology; 2020 Jun 1; 140:111312.
  4. Abbaszadegan A, Gholami A, Mirhadi H, Saliminasab M, Kazemi A, Moein MR. Antimicrobial and cytotoxic activity of Ferula gummosa plant essential oil compared to NaOCl and CHX: a preliminary in vitro study, Restorative dentistry & endodontics; 2015 Feb 1; 40(1):50-7.
  5. Ghahremaninejad F, Hoseini E. Identification of Medicinal and Aromatic Plants of Iran, Valiollah Mozaffarian. Farhang Moaser Publishers, Tehran (2012). 1444 pp., 2470 colored images (Language: Mainly Persian with English preface and several indexes). Hardback, ISBN: 978-600-1050-31-2. Format: 23.5× 15.5 cm. Price: 900000 IR-Rials. 10.1016/j.jep.2015.01.053
  6. Sayah M, Kamalinezhad M, Roustaeian AA, BAHRAMI HR. Antiepileptic potential and composition of the fruit essential oil of Ferula gummosa boiss.
  7. Khalifaev PD, Sharopov FS, Safomuddin A, Numonov S, Bakri M, Habasi M, Aisa HA, Setzer WN. Chemical composition of the essential oil from the roots of Ferula kuhistanica growing wild in Tajikistan, Natural Product Communications; 2018 Feb; 13(2):1934578X1801300226.
  8. da Silva Rivas AC, Lopes PM, de Azevedo Barros MM, Costa Machado DC, Alviano CS, Alviano DS. Biological activities of α-pinene and β-pinene enantiomers, Molecules; 2012 May 25; 17(6):6305-16.
  9. Valinezhad N, Talebi AF, Alamdari S. Biosynthesize, physicochemical characterization and biological investigations of chitosan-Ferula gummosa essential oil (CS-FEO) nanocomposite, International Journal of Biological Macromolecules; 2023 Jun 30; 241:124503.
  10. Sybilska D, Kowalczyk J, Asztemborska M, Ochocka RJ, Lamparczyk H. Chromatographic studies of the enantiomeric composition of some therapeutic compositions applied in the treatment of liver and kidney diseases, Journal of Chromatography A; 1994 Apr 8; 665(1):67-73.
  11. Alma MH, Nitz S, Kollmannsberger H, Digrak M, Efe FT, Yilmaz N. Chemical composition and antimicrobial activity of the essential oils from the gum of Turkish pistachio (Pistacia vera L.), Journal of agricultural and food chemistry; 2004 Jun 16; 52(12):3911-4.
  12. Zhou JY, Tang FD, Mao GG, Bian RL. Effect of alpha-pinene on nuclear translocation of NF-kappa B in THP-1 cells, Acta Pharmacologica Sinica; 2004 Apr 1; 25(4):480-4.
  13. Abd Alkareem, T., Hassan, S., Abdalhadi, S. Breast Cancer: Symptoms, Causes, and Treatment by Metal Complexes: A Review. Advanced Journal of Chemistry-Section B: Natural Products and Medical Chemistry, 2023; 5(4): 306-319. doi: 10.48309/ajcb.2023.398122.1174
  14. Atashbar E, pyman A Comparative Study of Extraction Methods and Components Identification of Teucrium Chamaedrys and Investigation of Their Antibacterial Effect, Progress in Chemical and Biochemical Research; 2021; 4(3):348-358.
  15. Adams RP. Identification of essential oil components by gas chromatography/mass spectrometry. 5 online ed. Gruver, TX USA: Texensis Publishing. 2017.
  16. Eftekhar F, Yousefzadi M, Borhani K. Antibacterial activity of the essential oil from Ferula gummosa seed, Fitoterapia; 2004 Dec 1; 75(7-8):758-9.
  17. Mortazaien F, Sadeghian MM. Investigation of Compounds from Galbanum (Ferula gummosa) Boiss, Asian Journal of Plant Sciences; 2006; 5(5):905-906. 10.3923/ajps.2006.905.906
  18. Talebi Kouyakhi E, Naghavi MR, Alayhs M. Study of the essential oil variation of Ferula gummosa samples from Iran, Chemistry of Natural Compounds; 2008 Jan; 44:124-6.
  19. Jalali HT, Petronilho S, Villaverde JJ, Coimbra MA, Domingues MR, Ebrahimian ZJ, Silvestre AJ, Rocha SM. Assessment of the sesquiterpenic profile of Ferula gummosa oleo-gum-resin (galbanum) from Iran. Contributes to its valuation as a potential source of sesquiterpenic compounds, Industrial Crops and Products; 2013 Jan 1; 44:185-91.
  20. Meshkatalsadat M, Salhvarzi S, Aminirad R. Chemical Composition of Volatile Oil from Ferula gummosa using Hydrodistillation Profile, MH; 2013; 29(1).
  21. Ghannadi A, Amree S. Volatile oil constituents of Ferula gummosa Boiss. from Kashan, Iran, Journal of Essential Oil Research; 2002 Nov 1; 14(6):420-1.
  22. Kanani MR, Rahiminejad MR, Sonboli A, Mozaffarian V, Kazempour Osaloo S, Nejad Ebrahimi S. Chemotaxonomic significance of the essential oils of 18 Ferula species (Apiaceae) from Iran, Chemistry & biodiversity; 2011 Mar; 8(3):503-17.
  23. Noma Y, Asakawa Y. Biotransformation of monoterpenoids. 10.1016/B978-008045382-8.00742-5
  24. Winnacker M. Pinenes: Abundant and renewable building blocks for a variety of sustainable polymers, Angewandte Chemie International Edition; 2018 Oct 26; 57(44):14362-71.
  25. Salehi B, Upadhyay S, Erdogan Orhan I, Kumar Jugran A, LD Jayaweera S, A. Dias D, Sharopov F, Taheri Y, Martins N, Baghalpour N, C. Cho W. Therapeutic potential of α-and β-pinene: A miracle gift of nature, Biomolecules; 2019 Nov 14; 9(11):738.
  26. Gargodhi, M., Rajab, F., Ansir, N. Evaluation of Cadmium (II) and Lead (II) in marine soil in Benghazi city using Atomic Absorption Spectroscopy. Advanced Journal of Chemistry-Section B: Natural Products and Medical Chemistry, 2023; 5(4): 320-329. doi: 10.48309/ajcb.2023.407758.1180
  27. Mercier B, Prost J, Prost M. The essential oil of turpentine and its major volatile fraction (α-and β-pinenes): a review, Int J Occup Med Environ Health; 2009 Jan 1; 22(4):331-42. 10.2478/v10001-009-0032-5
  28. da Silva Rivas AC, Lopes PM, de Azevedo Barros MM, Costa Machado DC, Alviano CS, Alviano DS. Biological activities of α-pinene and β-pinene enantiomers, Molecules; 2012 May 25; 17(6):6305-16.
  29. Özbek H, SEVER YILMAZ BE. Anti-inflammatory and hypoglycemic activities of alpha-pinene, ACTA Pharmaceutica Sciencia; 2017; 55(4).
  30. de Almeida Pinheiro M, Magalhães RM, Torres DM, Cavalcante RC, Mota FS, Coelho EM, Moreira HP, Lima GC, da Costa Araújo PC, Cardoso JH, de Souza AN. Gastroprotective effect of alpha-pinene and its correlation with antiulcerogenic activity of essential oils obtained from Hyptis species, Pharmacognosy magazine; 2015 Jan; 11(41):123.





Majid Halimi Khalilabad, Mohabat Nadaf. Chemical Profile of Ferula Gummosa Growing in the Saluk Protected Area of Iran and Comparing the Results with Other Habitats. Prog. Chem. Biochem. Res., 6(4) (2023) 277-291

DOI: 10.48309/pcbr.2023.410170.1271