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Document Type : Original Research Article


1 Department of Pure and Industrial Chemistry, Nnamdi Azikiwe University, Awka, Nigeria

2 Department of Chemistry, Federal University of Technology, Owerri, Nigeria

3 Directorate of Chemical Evaluation and Regulation, National Agency for Food, Drug Administration and Control, Lagos, Nigeria


 Phytochemical and antimicrobial analysis of Moringa oleifera seeds were carried using standard laboratory procedures. The phytochemical content showed the presence of alkaloid, flavonoid, saponin, and steroids respectively as samples contained 5.85g alkaloid, 5.97g flavonoid, and 1.46g saponin. Anti-microbial screening with certain selected human pathogens and the zones of inhibition obtained showed that alkaloids were 3mm, 3mm, 2mm, 4mm, & 5mm, flavonoids were 6mm, 5mm, 4mm, 2mm and 7mm and saponins were 4mm, 3mm, 3mm, 6mm and 5mm for Escherichia coli, Pseudomonas aureginosa, Klebsiella, Staphylococcus aureus and Streptocuccus aureus. Minimum inhibitory concentration (MIC) were 6.5mg/cm3, 12.5mg/cm3, 12.5mg/cm3, 6.5mg/cm3 and 12.5mg/cm3 for Escherichia coli, Pseudomonas aureginosa, Klebsiella, Staphylococcus aureus and Streptocuccus aureus respectively. This finding shows that the application of the seed extract of Moringa oleifera for potential therapeutic use and medicinal drugs in treatment of pneumonia, urinary tract infection and a host of other diseases in humans.

Graphical Abstract

Phytochemical and Anti-Microbial Analysis of Metabolites in seeds of Moringa oleifera grown in Nigeria


  • Moringa oleiferaexist majorly in the tropics
  • It has economic use in water treatment and production of perfumes and hair creams
  • It has nutritional uses in the formulation of food supplements and animal feeds
  • Phytochemical and antimicrobial assessment showed it has therapeutic use in treatment of diseases


Main Subjects

[1]           Y. Li, Y. Shen, C-L. Yao, and D-a. Guo. Quality assessment of herbal medicines based on chemical fingerprints combined with chemometrics approach: A review. Journal of Pharmaceutical and Biomedical Analysis. (2020), 113215
[2]           Xi-C. Wei, B. Cao, C-H. Luo, h-Z. Huang, P. Tan, X-R. Xu, R-C. Xu, M. Yang, Y. Zhang, L. Han, and D.-K. Zhang. Recent advances of novel technologies for quality consistency assessment of natural herbal medicines and preparations. Chinese Medicines. 15(1), (2020) 1-24
[3]           T. Ahmadu and K. Ahmad. An introduction to Bioactive natural products and general applications. Bioactive Natural Products for Pharamceutical Applications, 140,(2021) 41-91.
[4]           N.L. Shimanovskii. The role of phytoengineering in the preparation and production of herbal medicines.  Pharmaceutical chemistry. 53 (10), (2020), 961-963.
[5]           R.D.E, Sewell and M.R. Kopaei. The history and ups and downs of herbal medicines usage. Journal of Herbmed pharmaology. 3, (2014)
[6]           R. Mirzaeian, F. Sadoughi, S. Tahmasebian and M. Mojahedi. The role of herbal medicines in health care quality and the related challenges Journal of herbmed pharmacology. (2021).
[7]           B. Park., S. You, W.C.S. Cho, J-Y. Choi and M.S. Lee. A systematic review of herbal medicines for the treatment of cancer cachexia in animal models. Journal of Zhejiang University-Science B. 20(1), (2019) 9-22.
[8]           R. Luthfiyanti, A.C. Iwansyah, Y. Rahayu and N.S. Achyadi. Study of antioxidant activities acceptability and shelf life prediction of Ciplukan (Physalis angulate L.) juice drinks. IOP Conference Series: Materials Science and Engineering 1011 (1), (2021) 012001.
[9]           E. Verga, E. Becsek, S.G. Bartha, S. Stranczinger, F. Mihalovitis and N. Papp. Determination of polyphenols and invitro antimicrobial and antioxidant activity of Calluna vulgari (L.) Hull. Biologia Futura. (2021).
[10]       K. Sixl-Daniell, W. Sixl, G. Sixl and W. Fuchs. The use of Moringa oleifera as a medicinal plant in India and the Philippines. Fitomedicina.(2011), 1-5.
[11]       S.G. Zaku, S. Emmanuel, A.A. Tukur and A. Kabir. Moringha oleifera: An underutilized tree in Nigeria with amazing versatility: A review. African Journal of Food Science. 9(9), (2015) 456-461.
[12]       A. Ali, P. Garg, R. Goyal, G. Kaur, X. Li,  P. Negi, M. Valis K. Kuca, and S. Kulshrestha. A novel herbal hydrogel formulation of Moringha oleifera for wound healing. Plants.  10(1), (2021) 25.
[13]       R.J. Baquerizo-Crespo, Y. Nuñez, J. Albite, J.A. Macías-Alcívar, N. Cedeño-Zambrano, A.A. Dueñas-Rivadeneira, Y. Gómez-Salcedo, and J.M. Rodríguez-Díaz. Biocoagulants as an Alternative for Water Treatment. Advances in the Domain of Environmental Biotechnology (2021), 313-334.
[14]       Z.F. Ma, J. Ahmad, H. Zhang, I. Khan, and S. Muhammad. Evaluation of phytochemical and medicinal properties of Moringa (Moringa oleifera) as a potential functional food. South African Journal of Botany, 129 (2020) 40-46,
[15]       A. Tomaand S. Deyno. Phytochemistry and Pharmacological Activities of Moringa oleifera. Indian Journal of Pharmacology 1(4), (2014) 222-231.
[16]       K. Mangundayao and P. Yasurin. Bioactivity of Moringa oleifera and its applications: a review. Journal of Pure and Applied Microbiology. 11, (2017) 43-50.
[17]       A. Shaban, G.M. Mishra, R. Nautiyal, S. Srivastava, K. Tripathi, P. Chaudhary, S.K. Verma,. In vitro cytotoxicity of Moringa oleifera against different human cancer cell lines. Asian Journal Pharmaceutical Clinical Research, 5, (2012) 271-282.
[18]       L. Purwal, A.K. Pathakand U.K. Jain. In vivo anticancer activity of the leaves and fruits of Moringa oleifera on mouse melanoma. Pharmacology. 1, (2010) 655-658.
[19]       F.A.R. Ahmed, D.I. Muhammad, B.K. Saie, Health Benefits of Moringa oleifera. Asian Pacific Journal of Cancer Prevention. 15, (2014) 8571-8576.
[20]       E.I. Omodanisi, G.Y. Aboua and O.O. Oguntibeju. Therapeutic potentials and pharmacological properties of Moringa oleifera Lam in the treatment of diabetes mellitus and related complications. Tropical journal of pharmaceutical research 16 (7), (2017) 1737-1746
[21]       I.A. Raubilu, U. Isah,and M.A. Ahmad. Antimicrobial activity of Moringha oleifera: A short review. Bayero Journal of Pure and Applied Sciences. 12(1) 2019, 128-132
[22]       OA Eremwanarue and HO Shittu. Antimicrobial activity of Moringa oleifera leaf extracts on multiple drug resistant bacterial isolates from urine samples in Benin City. Nigerian Journal of Biotechnology 35 (2), (2018) 16-26.
[23]       A.R. Haneef and N. Kurup. Comparative Study of Fluoride Removal from Water by Using Moringa oleifera and Thulsi (Ocimum Sanctum). International Journal of Scientific and Engineering Research 7 (4), (2016) 222.
[24]       K. Francis and B. Amos. Effectiveness of Moringa oleifera seed as coagulant for water purification. African Journal of Agricultural Research. 4(1), (2009) 119-123.
[25]       S.N. Sinha, D. Paul and K. Biswas. Effects of Moringa oleifera Lam. and Azadirachta indica A. Juss. leaf extract in treatment of tannery effluent. Our Nature 14 (1), (2016) 47-53.
[26]       K.N. Kuben, M.C. Roger. Review on herbal remedies used by the 1860 South African Indian settler. African Journal of Biotechnology.10(43), (2011) 8533-8538.
[27]       S.O.O.M. Sali and T.S. Mohammed. The Role of Moringa oleifera as a Natural Antioxidants on Egg Laying Chicken Performance and Egg Quality. MSc. Abstract. Sudan University of Science and Technology, Sudan. (2018).
[28]       E.E. Akangbe. Utilisation of Moringa oleifera Lam. Seed as Protein Source in the Diets of Broiler Chicken. PhD Abstract, Animal Science Unit, Faculty of Agriculture and Forestry. University of Ibadan, Nigeria. (2017).
[29]       I.C. Iwu, M. Chijioke-okere, U.L. Onu and R. Uchegbu. GC-MS, Phytochemical and Antimicrobial analysis of the leaf of Newboudia laevis (P. Benth). International Journal of Innovative research development. 7(7), (2018) 242-250.
[30]       I.C. Iwu, U.L. Onu, A.A. Ukaoma and R.N. Oze. Phytochemical, Antimicrobial and GC/MS analysis of the root of Stachytarpheta Cayennensis (L.Vahl) grown in Eastern Nigeria. International Research Journal of Natural Science6(2), (2018) 1-14.
[31]       I.C., Iwu, I.U. Onu, M. Chijioke-Okere, A.A. Ukaoma and J.N. Azorji. GC-MS, Phytochemical and antimicrobial analysis of Pentaclethra macrophylla leaf. Int. J. Sci. Tech. 2016, 4 (7), 151-159.
[32]       I.A. Duru. Comparative Phytochemical Analysis of Brown, Green and Red Propolis from Umudike, Abia State Nigeria". Advanced Journal of Chemistry-Section B, 3(1),(2021) 86-97.
[33]       D. Dahiru, J.A. Onubiyi, and H.A. Umaru. Phytochemical screening and anti-ulcerogenic effect of Moringa oleifera aqueous leaf extract. African Journal of Traditional, Complementary and Alternative Medicines, 2006, 3(3), 70-75.
[34]       H. Zanrate, M. Susana, A. Juarez, M.D. Rosario, C.G. Abel, O.L. Cesar, G. Chavez, A. Josue, S. Garfias, J.D.J. Nezahualcoyotl and A.F. Ramos. Flavonoids, Phenolic content and antioxidant activity of propolis from various areas of Guanajuato. Mexico. Food Science and Technology. 38(2) (2018) 210-215.
[35]       I.A. Duru and C.E. Duru. Identification and Quantification of phytochemicals from Carica papaya Linn (Caricaceae) root extract using GC-FID.  Journal of Chemical Society of Nigeria. 44(7), (2019) 1291-1297.
[36]       P. Paliwal S.S. Pancholi and R.K. Patel. Pharmacognostic parameters for evaluation of the rhizomes of Curcuma caesia. Journal of Advanced Pharmaceutical Technology Research. 2(1), (2011) 56-61.
[37]       G-L. Chen, Y-B. Xu, J-L. Wu, N. Li and M-Q. Guo. Hypoglycemic and hypolipidemic effects of Moringa oleifera leaves and their functional chemical constituents. Food Chemistry, 333, (2020) 127478,
[38]       I. Ojeaga, H.G. Haruna and B. L.Tajudeen. Phytochemical and Antimicrobial Studies On Moringa Oleifera Leaves Extracts. Journal Of Environmental Science, Toxicology And Food Technology., 8(1),(2014) 39-45.
[39]       D.O. Omokpariola, V.U. Okechukwu and P.L. Omokpariola. Effect of processing on the nutritive and antinutritive properties of Afzelia africana.Advanced Journal of Chemistry- Section B, 3(2), (2021) 188-198.
[40]       V.U. Okechukwu, S.O. Eze, D.O.  Omokpariola and J.C. Okereke. Evaluation of physiochemical constituents of methanol extract of Moringa oleifera Lam whole leaf by gas chromatography – mass spectrometry and Fourier transformed infrared spectrometry. World News of Sciences, 37, (2021) 18-30.