Document Type : Original Research Article
Ph.D. in Biochemistry, Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran
Cellular and Molecular Research Center, Yasouj University of Medical Sciences, Yasouj, Iran
The use of medicinal herbs in the treatment of diseases has become increasingly widespread, in this regard the Verbena ofﬁcinalis is considered as one of the herbs that affects pregnancy. The present study was conducted to investigate the Verbena ofﬁcinalis herb on the level of sex hormones of NMRI female rats during pregnancy. This is an experimental study which divided 40 rats into 5 groups of 8 each and the groups included control, sham, and experimental with 5, 10, and 15 mM alcoholic extract of Verbena ofﬁcinalis. The serum levels measurement of FSH and LH hormones was done using ELISA (Biotech) method. After blood sampling, the ovaries were stained with hematoxylin eosin for histological examination. Cell count and thickness measurement of different ovarian cells, primitive follicles, primary follicles, growing follicles, graph follicles, lymph nodes, blood vessels, atretic follicles, and abnormal follicles (1 or 2 oocytes in one follicle) were examined microscopically in 6 segments of tissue segments from different groups. The statistical analysis was performed using SPSS23 software.
The findings of the present study showed that the consumption of alcoholic extract of Verbena ofﬁcinalis significantly reduced the levels of LH, FSH, the number of abnormal follicles, the number of graph follicles, and the number of corpus luteum in the experimental group compared to the control group (p≤0.05), the number of primary follicles, the number of growing follicles, the number of ethereal follicles, and the number of blood vessels in the experimental group compared to the control group (p≤0.05). The weight, ovarian weight, ovarian diameter, secondary follicle diameter, and primary follicle diameter decreased in experimental groups compared to control group and sham group; however, this decrease was not significant and abnormal follicle diameter in experimental groups compared to control group and group. Sham increased but this increase was not significant (p> 0.05). The results of the present study show that consumption of Verbena ofﬁcinalis medicinal plant can be effective in abortion
- Amirghofran, Medicinal plants as immunosuppressive agents in traditional Iranian medicine, Iranian J of Immun, 7 (2010) 65-73.
- Uma Devi, S. Murugan, S. Suja, S. Selvi, P. Chinnaswamy, E. Vijayanand, Antibacterial, In vitro Lipid per Oxidation and Phytochemical Observation on Achyranthes Bidentata Blume. Pakistan Journal of Nutrition, 6 (2007) 447-451.
- UÇAR TÜRKER, B. YÜCESAN, E. GÜREL, Adventitious shoot regeneration from stem internode explants of Verbena oicinalis L., a medicinal plant, Turk J Biol, 34 (2010) 297-304.
- R. Clapham, T.G. Tutin, D.M. Moore. Flora of British Isles. 3rd theEd. Cambridge University Press, Cambridge, (1987) 402.
- Russo, C.G. Galleti. Medicinal properties and chemical composition of Vitexagnus–castusthe crude alcoholic extract and flavonoids of vitexleucoxylon. Fitotropia, 61 (1990) 263–264.
- RC Wren. Potter's New Enyclopaedia of Botanical Drugs and Preparation, Saffron Walden The C.W. Daniel Company Limited, (1988) 275
- Mabey, The Complete New Herbal, Elm tree books, London, (1988) 128.
- Cossuta, B. Simndi, E. Vagi, J. Hohmann, A. PrechI, E. Lemberkovics, A. Kery, T. Keve, Supercritical fluid extraction of Vitexagnuscastus fruit, J Supercritical Fluids, 47 (2008) 188–94.
- Nasri, S.H. Oryan, A. HaeriRohani, H. Yahyavi, The effects of Vitexagnuscastus L. Extract on gonadotrophines and testosterone in male mice, Iranian Int J Sci, 5 (2004) 25-30.
- Ramezani, S. Nasri, H. Bahadoran, The effect of Vitexagnuscastus total extract on spermatogenesis of Balb/C mice, Armaghane-Danesh, 52 (2008) 354.
- Du Mee, Vitexagnuscastus, Aust J Med Herbalism, 5 (1993) 63-5.
- R. Farnsworth, A.S. Bingel, G.A. Cordell, F.A. Crane, H.H. Fong, Potential value of plants as sources of new antifertility agents I. J Pharm Sci, 64 (1975) 335-98.
- Azarnia, S. Ejtemaei-Mehr, A. Shakoor, A. Ansari, Effects of VitexAgnusCastud on mice fetus development, Acta Medica Iranica, 45 (2007) 263-70.
- UÇAR TÜRKER, B. YÜCESAN, E. GÜREL, Adventitious shoot regeneration from stem internode explants of Verbena oicinalis L., a medicinal plant, Turk J Biol, 34 (2010) 297-304
- Deepak, S.S. Handa, Antiinflammatory activity and chemical composition of extracts of Verbena officinalis, Phytotherapy Research, (2006) 12-19.
- Speroni, R. Cervellati, S. Costa, M.C. Guerra, A. Utan, P. Govoni, A. Berger, A. Müller, H. Stuppner, Effects of differential extraction of Verbena officinalis on rat models of inflammation, cicatrization and gastric damage. Planta Med., 73 (2007) 25-41.
- Shaukat, H. Shareef, M. Ahmad, S. Gouhar, G.H. Rrizwani, Pharmacognostic studies on fresh mature leaves of Holoptelea integrifolia (roxb) planch, Pak J Bot, 42 (2010) 3705-8.
- Fallah Huseini, S. Kianbakht, Study on effects of ch icory (Cichorium intybus L.), fennel (Foeniculum vulgare Mill.) and dill (Anethum graveolens L.) on fertility and neonatal gender in rats, J Med Plant, 11 (2012) 192-6
- Tendon, R.K. Gupta, Histomorphological changes induced by Vitexnegundo in albino rats, Indian J Pharmacol, 36 (2004) 175-80
- J. Dugoua, D. Seely, D. Perri, G. Koren, E. Mills, Safety and efficacy of chastetree (VitexAgnus-Castus) during pregnancy and lactation, J Clin Pharmacol, 15 (2008) e74-9.
- Ohyama, T. Akaike, C. Hirobe, T. Yamakawa, Cytotoxicity and apoptotic inducibility of VitexagnuscastusFruit extract in cultured human normal and cancer cells and effect on growth. Biol Pharm Bull, 26 (2003) 10-8.
- Hoberg, J.E. Orjala, B. Merier, O. Sticher, Diterpenoids from the fruits of vitex agnus–castus, Phytomed, 52 (1999) 1555-8.
- P. Schröder-van der Elst, D. van der Heide, H. Rokos, G. Morreale de Escobar, J. Köhrle, Synthetic flavonoid cross the placenta in the rat and are found in fetal brain, Am J Physiol, 274 (1998) E253-6.