Document Type : Original Research Article


1 Department of Biology, Faculty of Science, Shiraz University, Shiraz. Iran

2 General Practitioner, School of Medical sciences, Tehran Branch, Islamic Azad University, Tehran, Iran


In this study 'Therapeutic Effects of Hydro-alcoholic Extract of Papaver Rhoeas on Cardiovascular side effect of MIA Induced Osteoarthritis in male Rat is investigated. OA model in 35 male rats was created by injection of monosodium iodoacetate(MIA) into the right knee joint and then gavage with extract of Papaver Rhoeas for14 days, on day 30 The veins and arteries of the animal were then for injection and then Blood pressure parameters (systolic, diastolic, mean arterial pressure) were recorded and analysis effect of cholinergic and niterergic system .The results were analyzed by SPSS software. The effect of oral extracts of Papaver Rhoeas (200 mg/kg Dosage) on Systolic, diastolic, mean arterial pressure (mmHg) and heart rate (Beat/min) reduced compared with negative group (P <0/05). According to findings, extract of Papaver Rhoeas has a decreasing effect on blood pressure in rats with OA and have been observed at 200mg/kg doses, the least joint damage and the best physiological function of the heart. This effect may be due to inhibition of the niterergic and cholinergic system.

Graphical Abstract

Therapeutic Effects of Hydro-alcoholic Extract of Papaver Rhoeas on Cardiovascular side effect of MIA Induced Osteoarthritis in male Rat


Main Subjects

[1].  Hosseini S E, Hamzavi S, Aghababa H. The effects of alcoholic extract of red poppy (Papaver Rhoeas ) on anxiety induced by elevated plus maze and the plasma corticosterone levels in adult male wistar rats. Bimonthly Sabzevar University of Medical Sciences, 22, (2015), 4-10
[2]. Osanloo  N, Najafi-Abedi A, Jafari F, Javid  F, Pirpiran M, Jafari M R M, et al. Papaver Rhoeas  L. Hydroalcoholic Extract Exacerbates Forced Swimming Test-Induced Depression in Mice. Basic and clinical neuroscience,7, (2016),195.
[3]. Sankhari, J. M., Thounaojam, M. C., Jadeja, R. N., Devkar, R. V., & Ramachandran, A. V. Anthocyanin‐rich red cabbage (Brassica oleracea L.) extract attenuates cardiac and hepatic oxidative stress in rats fed an atherogenic diet. Journal of the Science of Food and Agriculture, 92(8), (2012), 1688-1693
[4]. Fabule, J., & Adebajo, A. Comparative evaluation of cardiovascular outcomes in patients with osteoarthritis and rheumatoid arthritis on recommended doses of nonsteroidal anti-inflammatory drugs. Therapeutic advances in musculoskeletal disease, 6(4), (2014), 111-130.
[5]. Reddy M K, Schultz H D, Zheng H, Patel K P. Altered nitric oxide mechanism within the paraventricular nucleus contributes to the augmented carotid body chemoreflex in heart failure. American Journal of Physiology-Heart and Circulatory Physiology, 292, (2007),149-157
[6]. Mohammad Shokrzadeh, Emran Habibi, Mona Modanloo, Somaye Hesami,Cytotoxic Effect of Methanolic Extract of Papaver Rhoeas  L. on Vero Cell Line and Its Antioxidant Activity. Journal of Mazandaran University Medical Science, 26(145), (2017), 364-370 (Persian).
[7]. Gargiulo G, Capodanno D, Longo G, Capranzano P,Tamburino C. Updates on NSAIDs in patients with and without coronary artery disease: pitfalls, interactions and cardiovascular outcomes. Expert review of cardiovascular therapy, 12(10), (2014), 1185-1203.
[8].        Cassidy, A., Rogers, G., Peterson, J. J., Dwyer, J. T., Lin, H., & Jacques, P. F. Higher dietary anthocyanin and flavonol intakes are associated with anti-inflammatory effects in a population of US adults. The American journal of clinical nutrition, 102(1), (2015), 172-181.
[9].        Agnieszka P, Magdalena K, Natalia M, Michal K, Katarzyna S. Expression of matrix metalloproteinases and components of the endocannabinoid system in the knee joint are associated with biphasic pain progression in a rat model of osteoarthritis. Journal of Pain Resource, 10, (2017), 1973–1989.
[10].    Abass, A.M., Malallah Rzaij, J., A Review on: Molecularly Imprinting Polymers by Ion Selective Electrodes for Determination Drugs , Journal of Chemical Reviews,2(3), (2020), 148-156
[11].   Fereidoni M, Ahmadiani A, Semnanian S, Javan M. An accurate and simple method for measurement of paw edema. Journal of  Pharmacol Toxicol Methods, 43(1), (2000),11-14.
[12].   Chen, Y. F., Shibu, M. A., Fan, M. J., Chen, M. C., Viswanadha, V. P., Lin, Y. L., ... & Huang, C. Y. Purple rice anthocyanin extract protects cardiac function in STZ-induced diabetes rat hearts by inhibiting cardiac hypertrophy and fibrosis. The Journal of nutritional biochemistry, 31, (2016), 98-105.
[13].   Giorgio Gentile1, Fabio Angeli , Giovanni Mazzotta1, Gianpaolo Reboldi ,Paolo Verdecchia. Emerging therapies in the management of hypertensive patients with osteoarthritis, Clin. Invest, 1(1), (2011), 125-136
[14].   Huang, P. C., Kuo, W. W., Shen, C. Y., Chen, Y. F., Lin, Y. M., Ho, T. J., ... & Huang, C. Y. (). Anthocyanin attenuates doxorubicin-induced cardiomyotoxicity via estrogen receptor-α/β and stabilizes HSF1 to inhibit the IGF-IIR apoptotic pathway. International journal of molecular sciences, 17(9), (2016), 1588.
[15].   Wang H, Bai J, He B, Hu X, & Liu D. Osteoarthritis and the risk of cardiovascular disease: a meta-analysis of observational studies. Scientific reports, 6, (2016), 396-72.
[16].   Huston J M, Tracey K J. The pulse of inflammation: heart rate variability, the cholinergic anti‐inflammatory pathway and implications for therapy. Journal of internal medicine, 269(1), (2011), 45-53
[17].   Tanaka, K., Hamada, K., Nakayama, T., Matsuda, S., Atsumi, A., Shimura, T., & Nemoto, M. Risk for cardiovascular disease in Japanese patients with rheumatoid arthritis: a large-scale epidemiological study using a healthcare database. Springer Plus, 5(1), (2016), 1111.
[18].   Grimsholm, O., Rantapää-Dahlqvist, S., Dalén, T., & Forsgren, S. Unexpected finding of a marked non-neuronal cholinergic system in human knee joint synovial tissue. Neuroscience letters, 442(2), (2008), 128-133.
[19].   Moslehipour A., Recent Advances in Fluorescence Detection of Catecholamines, Journal of Chemical Reviews, 2(3), (2020), 130-147
[20].   Yin, W., Xu, H., Sheng, J., Xu, Z., Xie, X., & Zhang, C. Comparative evaluation of the effects of platelet‑rich plasma formulations on extracellular matrix formation and the NF‑κB signaling pathway in human articular chondrocytes. Molecular Medicine Reports, 15(5), (2017), 2940-2948.
[21].   H. Treviño-Cordero, L. G. Juárez-Aguilar, D. I. Mendoza-Castillo, V. Hernández-Montoya, A. Bonilla-Petriciolet, and M. A. Montes-Morán, Synthesis and adsorption properties of activated carbons from biomass of Prunus domestica and Jacaranda mimosifolia for the removal of heavy metals and dyes from water, Industrial Crops and Products, 42, (2013), 315-323.