Hematology and Serum Biochemical Indices of Growing Rabbits Fed Diet Supplemented with Different Level of Indigofera Zollingeriana Leaf Meal

John, Alagbe; Oluwafemi, R.A

doi:10.33945/SAMI/PCBR.2019.4.2

Document Type : Original Research Article

Authors

Alagbe John ¹
R.A Oluwafemi ²

¹ Department of Animal Nutrition, Sumitra Research Farm, Gujarat, India

² Department of Animal Science, Faculty of Agriculture, University of Abuja. Nigeria

10.33945/SAMI/PCBR.2019.4.2

Abstract

This study was conducted to determine the hematological and serum biochemical parameters of growing rabbits fed diet supplemented with Indigofera zollingeriana leaf meal (IZM). A total number of Fifty (50) growing rabbits of between seven and eight weeks of age were randomly assigned to five treatment groups in a completely randomized design. Each group was replicated five times with two rabbits per replicate. Five experimental diets were formulated such that T1, T2, T3, T4 and T5 was supplemented with IZM at 0g, 2.5g, 5.0g, 7.5g and 10g respectively. Feed and water were provided ad libitum and the experiment lasted for 8 weeks. The hematological parameters determined included pack cell volume (PCV), hemoglobin (Hb), red blood cells (RBC), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), white blood cell (WBC) and its differentials while those of serum biochemical indices are: total protein, globulin, albumin, cholesterol, serum glutamic pyruvate transaminase (SGPT), serum glutamic oxaloacetate transaminase (SGOT). Results on hematology revealed that there were no significant differences (P>0.05) in the values of PCV, RBC, MCV, WBC and its differentials. Hb, MCH and MCHC values were significantly (p <0.05) influenced by the dietary supplementation of IZM. All results on the serum biochemical parameters showed that there was no significant (P>0.05) differences among the treatments. It was concluded that dietary supplementation of IZM up to 10g/kg had no deleterious effect on the hematology and serum biochemical parameters of rabbits.

Graphical Abstract

Keywords

20.1001.1.26767090.2019.2.4.2.5

Main Subjects

Biochemical and Medical science

Full Text

References

References:

[1] C. Opara, Studies on the use of Alchornia cordifolia leaf meal as feed ingredient in poultry diets. International Journal of Poultry Science, 9 (1996) 363-367.

[2] D. Brugger and W.M. Windisch, Environmental responsibilities of livestock feeding using trace mineral supplements. Animal Nutrition, 1 (2015) 113-118.

[3] S. Chandrasekaran, PHYTOCHEMICAL ANALYSIS AND ANTIBACTERIAL AND ANTIOXIDANT PROPERTIES OF INDIGOFERA TINCTORIA L. Asian Journal of Pharmaceutical and Clinical Research, 11 (2018)

[4] A. Ali, L. Abdullah, P. Karti, M. Chozin and D. Astuti, Production and nutritive value of Indigofera zollingeriana and Leucaena leucocephala in peatland. Animal Production, 16 (2015) 156-164.

[5] V. Maikai, U. Abubakar, A. Salman and T. Inuwa, Preliminary Survey of Medicinal Plants Used in Treatment of Animal Trypanosomosis in Kaduna State, Nigeria. Ethnobotanical Leaflets, 2010 (2010) 9.

[6] M.M. Cowan, Plant products as antimicrobial agents. Clinical microbiology reviews, 12 (1999) 564-582.

[7] M.U. Dahot, Antibacterial and antifungal activity of small protein of Indigofera oblongifolia leaves. Journal of ethnopharmacology, 64 (1999) 277-282.

[8] N.C. Jain, Schalm's veterinary hematology. (1986): Lea & Febiger.

[9] B. Bush, Interpretation of laboratory results for small animal clinicians. (1991): Blackwell Scientific Publications Ltd.

[10] L. Roschian, E. Bernat and W. Grubber, Enzmtrache bestimung des gesamtcholestrins in serus. Z Klin Chem Klin Biochem, 12 (1974) 403-407.

[11] S. Reitman and S. Frankel, A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. American journal of clinical pathology, 28 (1957) 56-63.

[12] AOAC, Official methods of analysis. 2nd edition ed. Official methods of analysis. Association of analytical chemists. . (2000), Washington, D.C.

[13] A. Harbone, Phytochemical methods: a guide to modern technique of plant analysis Chapman Hall. New York, (1973)

[14] O. Odebiyi and E. Sofowora, Phytochemical screening of Nigerian medicinal plants II. Lloydia, 41 (1978) 234-246.

[15] N.R. Council, Nutrient Requirements of Rabbits: 1977. (1977): National Academies Press.

[16] L. Abdullah, D. Apriastuti and T. Apdini. Use of Indigofera zollingeriana as a forage protein source in dairy goat rations. in Proc. Asia dairy Goat Conference Malaysia. 2012.

[17] T.A. Abidemi, O.J. Adebayo, O. Idowu and M. Agbotoba, Nutrient content and anti-nutritional factors in shea butter (Butryospermum parkii) leaves. African Journal of Biotechnology, 8 (2009)

[18] M. Özcan, Mineral contents of some plants used as condiments in Turkey. Food chemistry, 84 (2004) 437-440.

[19] H.A. Harper, R.K. Murray, D.K. Granner and P.A. Mayes, Harper's biochemistry. (2000): McGraw-Hill Health Professions Divisions.

[20] WHO, Trace elements and other essential nutrients. Clinical Application of Tissue Mineral Analysis. (1996: USA. 182.

[21] A. Munro and O. Bassir, Oxalate in Nigerian vegetables. West African Journal of Biology and Applied Chemistry, 12 (1969) 4-18.

[22] A. Musa, G. Abbas, A. Aliyu, M. Abdullahi and I. Akpulu, Phytochemical and antimicrobial screening of Indigofera conferta Gillett (Papilionaceae). Res J Med Plant, 2 (2008) 74-78.

[23] T.U. Rahman, M.A. Zeb, W. Liaqat, M. Sajid, S. Hussain and M.I. Choudhary, Phytochemistry and Pharmacology of Genus Indigofera: A Review. Records of Natural Products, 12 (2018) 1.

[24] V. Kumar, A.K. Sinha, H.P. Makkar and K. Becker, Dietary roles of phytate and phytase in human nutrition: A review. Food Chemistry, 120 (2010) 945-959.

[25] A.F. Ugadu, M. Ominyi, M. Ogbanshi and U. Eze, Phytochemical Analysis of Spondias Mombin. International Journal of Innovative Research and Development, 3 (2014) 101-107.

[26] T.B. Poole and R. Robinson, UFAW handbook on the care and management of laboratory animals. (1987): Longman.

[27] I. Archetti, C. Tittarelli, M. Cerioli, R. Brivio, G. Grilli and A. Lavazza. Serum chemistry and hematology values in commercial rabbits: preliminary data from industrial farms in northern Italy. in Proceedings of 9th World Rabbit Congress, Italy. 2008.

[28] F.L. Tavares, M.C.C. Sousa-e-Silva, M.L. Santoro, K.C. Barbaro, I.M.M. Rebecchi and I.S. Sano-Martins, Changes in hematological, hemostatic and biochemical parameters induced experimentally in rabbits by Loxosceles gaucho spider venom. Human & experimental toxicology, 23 (2004) 477-486.

[29] C. Özkan, A. Kaya and Y. Akgül, Normal values of haematological and some biochemical parameters in serum and urine of New Zealand white rabbits. World Rabbit Science, 20 (2012) 253-259.

[30] J.R. Jenkins, Rabbit diagnostic testing Journal of Exotic Pet Medicine, 17 (2008) 4-15.

[31] L. Isaac, G. Abah, B. Akpan and I. Ekaette. Haematological properties of different breeds and sexes of rabbits. in Proceedings of the 18th Annual Conference of Animal Science Association of Nigeria. 2013.

[32] T. Ahemen, A. Abu and V. Gbor, Haematological and serum biochemical parameters of rabbits fed varying dietary levels of water spinach Ipomoea aquatic leaf meal. Adv Appl Sci Res, 42 (2013) 370-373.

[33] O. Adua, I. Akinmuyisitana and F. Gbore, Growth performance and blood profile of female rabbits fed dietary cerium oxide. Journal of Bio-Science, 21 (2013) 69-75.

[34] P. Jiwuba, K. Ikwunze, E. Dauda and D. Ugwu, Haematological and serum biochemical indices of growing rabbits fed diets containing varying levels of Moringa oleifera leaf meal. British Biotechnology Journal, 15 (2016) 1-7.

[35] M. Elmas, E. Yazar, K. Uney and A. Karabacak, Pharmacokinetics of flunixin after intravenous administration in healthy and endotoxaemic rabbits. Veterinary research communications, 30 (2006) 73-81.

[36] T.d.O. Silva, L.C. Kreutz, L.J.G. Barcellos, J. Borella, A.B. Soso and C. Souza, Reference values for chinchilla (Chinchilla laniger) blood cells and serum biochemical parameters. Ciência Rural, 35 (2005) 602-606.

[37] S. Taghavi Fardood, A. Ramazani, M. Ayubi, F. Moradnia, S. Abdpour and R. Forootan, Microwave Assisted Solvent-free Synthesis of 1-phenyl-1, 2-dihydro-3H-naphtho[1, 2-e][1, 3]oxazin-3-one Catalyzed by FeCl3. Chemical Methodologies, 3 (2019) 583-589.

[38] F. Mangi, F.A. Sattar and C. Zhong Lu, Metal Free Synthesis of Organosulfur Compounds Employing Eosin Y as Photoredox Catalyst. Chemical Methodologies, 3 (2019) 599-606.

[39] R. Alizadeh and N. Ghazinia, Synthesis, Experimental and Theoretical Characterization of Co (III) Complexes of 2-Hydroxynaphthaldehyde. Advanced Journal of Chemistry, Section A: Theoretical, Engineering and Applied Chemistry, 2 (2019) 184-189.

[40] M.A. Mohamed, Simultaneous Determination of Amlodipine Besylate, Valsartan, and Its Related Substances in Their Film-Coated Tablets Dosage form by RP-HPLC Method. Advanced Journal of Chemistry, Section A: Theoretical, Engineering and Applied Chemistry, 2 (2019) 335-346.

[41] A.A. Dar, A.K. Dwivedi, P.K. Iyer and A. Khan, Indole derived “turn-on” fluorometric probe for dual detection of Hg2+ and Cu2+ ions at nanomolar level. Asian Journal of Green Chemistry, 2 (2018) 171-180.

[42] P. Amudha, H. Famitha, V. Selvaraj, M. Sathiyaraj, P. Venkatesh and V. Rajendran, Propargylation of indole under a new dual-site phase-transfer catalyst: A kinetic study. Asian Journal of Green Chemistry, 2 (2018) 201-216.

[43] O. Byanet, S. Adamu, S. Salami and H. Obadiah, Haematological and plasma biochemical parameters of the young grasscutter (Thyronomys swinderianus) reared in northern Nigeria. Journal of cell and Animal Biology, 2 (2008) 177-181.

[44] N. Pagrut, S. Ganguly and P. Yadav, Haematology of weaner rabbits fed diets containing culture fermented cowpea husk. (2018)

[45] Z. Rezayati Zad, S.S. Hosseiny Davarani, A.R. Taheri and Y. Bide, Highly selective determination of amitriptyline using Nafion-AuNPs@ branched polyethyleneimine-derived carbon hollow spheres in pharmaceutical drugs and biological fluids. Biosensors and Bioelectronics, 86 (2016) 616-622.

[46] R. Jalilian and A. Taheri, Synthesis and application of a novel core-shell-shell magnetic ion imprinted polymer as a selective adsorbent of trace amounts of silver ions. e-Polymers, 18 (2018) 123-134.

[47] M. Afrasiabi, S. Kianipour, Z. Rezayati Zad, B. A and T. A, A sensor for determination of tramadol in pharmaceutical preparations and biological fluids based on multi-walled carbon nanotubes-modified glassy carbon electrode. Journal of the Chemical Society of Pakistan, 35 (2013) 1106-1112.

How to cite this manuscript:

A. John and R.A. Oluwafemi, Hematology and Serum Biochemical Indices of Growing Rabbits Fed Diet Supplemented with Different Level of Indigofera Zollingeriana Leaf Meal. Progress in Chemical and Biochemical Research, (2019) 170-177.

Hematology and Serum Biochemical Indices of Growing Rabbits Fed Diet Supplemented with Different Level of Indigofera Zollingeriana Leaf Meal

Full Text

Full Text

References

References

Volume 2, Issue 4November 2019Pages 170-177

Volume 2, Issue 4
November 2019
Pages 170-177