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Document Type : Original Research Article


1 Dept. Chemistry, Benue State Unversit, Makurd-Ngera

2 Dept. Chemistry, Benue State Unversity, Makurrdi-Ngeria



This research reports the novel bioactivities of neem leaves powder, neem leaves concentrate, neem seeds powder and neem seeds oil against the weevil (Callosobruchus maculatus) on bambara groundnut. The leaves were grounded, crushed and subjected to ethanol to get the concentrate. While the seeds were dried under shade for one week, ground into fine particles. Cold bulk extraction was then used to obtain the neem seeds oil. The mortality count of Callosobruchus maculatus (C. maculatus) was studied by exposing the weevil to neem treated bambara groundnut at different days under laboratory condition. The neem dosage were 0.0, 0.1, 0.5 1.0 g/10 g of bambara groundnut. The percentage weight loss and seed damage were also determined at the end. The results indicated that neem seeds oil and neem leaves concentrate provided effective and comparable mortality counts against the C. maculatus. Again the neem powder (seeds and leaves) mortality counts were also comparable, but were less effective against the weevils as compared to the neem oil and concentrate at least for the first two days. Therefore, these neem materials could be used as biopesticides at the dose of _0.1 per 10 g to prevent C. maculatus attack on bambara groundnut during storage. This will reduce the usage of synthetic pesticides, thereby preventing their adverse residual effects on human and the environment.

Graphical Abstract

The bioactivities of the neem (seeds and leaves) against Callosobruchus maculatus on a Vigna Subterranean L.


Main Subjects

[1] L.S.N. Tinkeu, C. Madou, W. Djakissam, A. Goudoum and R. Ndjouenkeu,Post-harvest storage systems and insect pests occuring on Bambara groundnuts (Vigna subterranean (L.) Verde) in the Sudano-Guinean savannah of Cameroon. J. Entomol. Zool. Stud, 4 (2016),  167-173.
[2] R. Uddin II, K. Azeez and A. Lawal,Potentials of two bio-pesticides in the control of some field insect pests of bambara groundnut in Ilorin. Ethiopian Journal of Environmental Studies and Management, 10 (2017),  443-450.
[3] N. Aviara, A. Lawal, A. Atiku and M. Haque,Bambara groundnut processing, storage and utilization in north eastern Nigeria. Cont J Eng Sci, 8 (2013),  28-36.
[4] M.D. Moreira, M.C. Picanço, L.C.d.A. Barbosa, R.N.C. Guedes, M.R.d. Campos, G.A. Silva and J.C. Martins,Plant compounds insecticide activity against Coleoptera pests of stored products. Pesquisa Agropecuária Brasileira, 42 (2007),  909-915.
[5] R. Pavela,History, presence and perspective of using plant extracts as commercial botanical insecticides and farm products for protection against insects–a review. Plant Protection Science, 52 (2016),  229-241.
[6] B. Joseph and S. Sujatha,Insight of botanical based biopesticides against economically important pest. International Journal of Pharmacy & Life Sciences, 3 (2012),  2138-2148.
[7] W.M. Hikal, R.S. Baeshen and H.A.H. Said-Al Ahl,Botanical insecticide as simple extractives for pest control. Cogent Biology, 3 (2017),  1404274.
[8] R. Peshin and A.K. Dhawan, Integrated Pest Management: Volume 1: Innovation-Development Process. Vol. 1. (2009): Springer Science & Business Media.
[9] E. Mondal and K. Chakraborty,Azadirachta indica-A Tree With Multifaceted Applications: An Overview. Journal of Pharmaceutical Sciences and Research, 8 (2016),  299.
[10] O. Aremu, M. Femi-Oyewo and K. Popoola,Repellent action of Neem (Azadirachta indica) seed oil cream against Anopheles gambiae mosquitoes. African Research Review, 3 (2009).
[11] B. Sisay, T. Tefera, M. Wakgari, G. Ayalew and E. Mendesil,The Efficacy of Selected Synthetic Insecticides and Botanicals against Fall Armyworm, Spodoptera frugiperda, in Maize. Insects, 10 (2019),  45.
[12] A.M.A. K. and S.S. K. J. Environ. Sci. Nat. Resour., 10 ( 2017),  137–14.
[13] C. Regnault-Roger, C. Vincent and J.T. Arnason,Essential oils in insect control: low-risk products in a high-stakes world. Annual review of entomology, 57 (2012),  405-424.
[14] Z.R. Zad, S.S.H. Davarani, A. Taheri and Y. Bide,A yolk shell Fe3O4@ PA-Ni@ Pd/Chitosan nanocomposite-modified carbon ionic liquid electrode as a new sensor for the sensitive determination of fluconazole in pharmaceutical preparations and biological fluids. Journal of Molecular Liquids, 253 (2018),  233-240.
[15] K. Ileke and M. Oni,Toxicity of some plant powders to maize weevil, Sitophilus zeamais (Motschulsky)[Coleoptera: Curculiondae] on stored wheat grains (Triticum aestivum). Afr. J. Agric. Res, 6 (2011),  3043-3048.
[16] S. Achio, E. Ameko, F. Kutsanedzie and S. Alhassan,Insecticidal effects of various neem preparations against some insects of agricultural and public health concern. International Journal of Research in BioSciences, 1 (2012),  11-19.
[17] M. Su and M. Mulla,Activity and biological effects of neem products against arthropods of medical and veterinary importance. Journal of the American Mosquito Control Association, 15 (1999),  133-152.
[18] O.R. Pitan, A.T. Kehinde, A.A. Osipitan, A. Ademolu and O.A. Lawal,Laboratory Evaluation of Insecticidal Activities of Some Botanicals against Four Insect Pests of Honey Bees (Apis mellifera L.). International Journal of Applied Agriculture and Apiculture Research, 11 (2015),  172-182.
[19] K.O. Boadu, S.K. Tulashie, M.A. Anang and J.D. Kpan,Production of natural insecticide from Neem leaves (Azadirachta indica). Asian J. Plant. Sci. Res, 1 (2011),  33-8.
[20] E. Edelduok, E. Akpabio, J. Eyo and E. Ekpe,Evaluation of the insecticidal activities of cotyledon powder of melon, Citrullus vulgaris Schrad against the maize weevil, Sitophilus zeamais (Motsculsky). Journal of Biopesticides and Environment, 1 (2015),  50-57.