The emerging field of carbon based allotropes such as graphene, multiwall and singlewall nanotubes exhibits important role in field of electronics, optoelectronics, electrochemical and biomedical applications due to its unique structure and remarkable electronic, optical, thermal, mechanical and electrical properties[1-3]. One of the promising candidate among the allotropes of carbon is graphene is used for electrochemical sensing [4, 5], since every atom in a graphene sheet is a surface atom, molecular interaction and thus electron transport through graphene can be highly sensitive to adsorbed molecules . Although various methods have been developed to prepare individual graphene sheets, such as chemical vapour deposition (CVD), micromechanical exfoliation of graphite and epitaxial growth on electrically insulating surfaces, chemical reduction of graphite oxide colloidal suspensions has been considered as an effective route to synthesize graphene sheets due to its simplicity, reliability, ability for large-scale production and cost effectiveness [7-9]. Graphite oxide consists of a layered structure of ‘graphene oxide (GO)’ sheets bearing a strongly oxygenated, highly hydrophilic layered material that can be readily exfoliated in water to yield stable dispersions consisting mostly of single layer, which are referred to as graphene oxide [10-14]. At present chemically converted graphene by the modified Hummers’ method is the most widely used technique for preparing GO with excellent processability [15-17] which involves harsh oxidation of graphite to introduce abundant hydrophilic oxygenated functional groups.
Dopamine (DA) and Uric Acid (UA) plays vital roles in physiological functions of mammalian. Dopamine one of the most important catecholamine neurotransmitter widely distributed in cardiovascular, hormonal, renal and central nervous system . Uric Acid is primary end product of metabolic breakdown of purine. Any abnormal change of both the biomolecules leads to cause for several diseases, such schizophrenia, Alzheimer’s disease and parkison’s disease in case of dopamine abnormalities. On other hand, abnormal concentration levels of uric acid can cause gout, pneumonia, leukemia and hyperuricaemia[20-22]. Usually, dopamine coexists with uric acid in physiological samples which make major challenge in selectivity and sensitivity. Furthermore, their oxidation potentials are close to each other. To overcome these setbacks, we develop an rGO modified GC electrode for simultaneous electro-chemical detection of dopamine and uric acid. Modified electrode shows well defined peak separation and low limit of detection for DA and UA with good stability and reproducibility. Particularly, these factors suggest that the proposed sensor could be applied for determination of DA and UA in real sample analysis.
2. Materials and methods
2.1 Graphite oxide preparation (GO)
GO was synthesized from natural graphite powder by a modified Hummers method. Briefly, 2 g of natural graphite powder and 1 g of NaNO3 was added into a mixture of 70 ml of 98% concentrated sulfuric acid (Conc.H2SO4). The mixture was stirred in an ice bath for 30 minutes at temperature below 20°C and then, 6g of potassium permanganate was added to the suspension very slowly and stirred for 3 hrs and maintained the temperature below 20°C. After that, the suspended solution was transferred to water bath and stirred at 35°C for one hour. The mixture turns out dark green color in appearance. Then, 150 ml of deionized (DI) water was added slowly and stirred for 40 minutes at 980 C while the mixture turned dark brownish in color. After dilution with additional 240 ml DI water and followed by 5ml of Hydrogen Peroxide (H2O2, 30% concentration)was added to the mixture to terminate the reaction and stirring for another 2 hours changing the color of the mixture from the dark brown to brilliant yellow. The resulting mixture was washed repeatedly with 10% HCl and DI water to remove sulphate ions by centrifugation until pH attains 6. Then the final product was dried in an oven for 24 hours.
2.2 Reduced graphene oxide preparation (rGO)
400mg of graphite oxide powder was dispersed in 400 ml of DI water via sonication vigorously for 1 hour to exfoliate the graphite oxide into graphene oxide. Then, 5% of NaOH was added to obtain the pH value at 9. Thereafter, 4 g of NaBH4 was added to above dispersion and stirred for 1 hour. The resulting mixture was washed repeatedly with DI water by centrifugation, then dried at 50°C in an oven and the final product is collected and stored.
2.2.1 Preparation and modification of electrodes
Prior to modification, the bare Glassy carbon electrode GCE was polished carefully with 0.5µm alumina powder on polishing cloth and then rinsed ultrasonically in ethanol and water for 2 min, respectively. rGO was dispersed in deionized water (1mg/mL)by using ultrasonicator. Then, 10 μL of the prepared suspension was dropped onto the GCE surface by drop casting method and dried at room temperature for 1 hr to obtain the rGO modified GCE.
2.3 Characterization techniques
Fourier transform infrared (FTIR) spectra of the samples were recorded on a spectrophotometer (Perkin ElmerSpectrum RX I) using KBr as the mulling agent. Ultraviolet-Visible (UV-Vis) spectra of the samples were collected on a UV-Vis spectrophotometer (Perkin-Elmer Lambda 35), and X-ray diffraction analysis (XRD) of powders were carried out on XPERT-PROX-ray diffractometer with monochromatic CuKα, radiation (λ=1.5406 A°). Data were collected from 10° to 80° at a scan rate of 0.05° per 10.16 seconds. The morphologies of the samples were observed under scanning electron microscope (Carl Zeiss EVO 18). Cyclic voltammetry, electrochemical impedance spectroscopy and square wave voltammetry measurements were performed with a CHI 6005D workstation (CH Instruments,USA). A conventional three-electrode system was employed comprising a glassy carbon electrode (GCE, 0.07 cm2 in diameter) coated with rGO as the working electrode, a platinum wire as the auxiliary electrode, and an Ag/AgCl as the reference electrode.
3. Results and discussion
3.1 Structural characterization
3.1.1 X-ray diffraction:
XRD analysis is a non destructive technique to determine the phase formation, crystallinity of rGO sample as shown in the Fig. 1. Since XRD is not a perfect tool for identifying single-layer graphene, but it is very informative. The inset figure shows pristine graphite exhibits a basal reflection (002) peak at 2𝜃 = 25.5 o (d spacing = 0.349 nm) in the XRD pattern. After oxidation, graphite oxide (GO) shows a well-defined diffraction peak at 2θ=11o with the interlayer spacing (d-spacing) corresponding to 0.8 nm which is very typical for GO . This is an evidence to show that graphite is converted to graphene oxide. An increase in interlayer spacing of graphene oxide could be attributed to the intercalation of the oxygen functional groups like hydroxyl, epoxy and carbonyl as well as water molecules between graphene layers during the process of oxidation .
Also there is a very weak diffraction peak at 2𝜃 = 42.2°, which is believed due to the incomplete oxidation. After the reduction with NaBH4, the diffraction peak at (11°) of GO was disappeared and a very weak and broad diffraction peak can be observed in the XRD pattern of rGO at 2𝜃 = 18°–25°. This implies that the distance between the graphene of the oxygen and other functional
Fig. 1. X-Ray diffraction patterns Graphite (Inset), Graphite Oxide and rGO.
groups and restoration of carbon atoms in graphite network after the chemical reduction of NaBH4. Thus from the XRD results, it can be concluded that both epoxide and carboxylic functional groups were effectively reduced by sodium borohydride in line with the previous reports .
3.2. Morphological characterization
3.2.1 SEM surface morphology
Fig. 2 shows typical results of the studies of reduced graphene oxide. Fig 2(a) represents the view of the sample at lower magnification which stands for examining the area of 800×800 μm2 surface. Around the examined area, one can notice the presence of objects of sizes within 200 μm to 300 μm. Those objects consist of tiny particles, as can be proved by SEM studies results gathered on one of the particles. The above results are shown in Fig. 2(b) (25×25 μm2 area, higher magnification). It is easy to notice that the examined particles consist of a number of smaller objects of 0.5 μm to few micrometers in size. The problem was caused by the factor responsible for difficulties connected with getting higher magnification were high susceptibility of nanoparticles to aggregate into larger conglomerates. It should be pointed here that, except for nanoparticles
Fig. 2.SEM images of rGO (a) Lower magnification (800×800 μm2) (b) Higher magnification (25×25 μm2).
conglomerates, in GO powder there may appear stable grain of size ranging even to single micrometers.
3.2.2 EDAX Analysis
Fig. 3. shows a standard EDAX spectrum recorded to determine the elemental compositions of the sample. In the middle part of the presented spectrum one can clearly see three peaks located between 0.1kV and 1.2 kV. Those maxima are directly related to the GO characteristic lines K and L. The maximum located on the left part of the spectrum at 0.2 kV clearly comes from carbon. The hardly visible maximum located at 0.5 keV is connected with the oxygen characteristic line. The carbon and oxygen spots in the examined samples confirm the presence of stabilizers composed of alkyl chains. The spectra obtained during EDAX studies were used for carrying out the quantitative analysis. For that purpose, SEM Quant software and the ZAF procedure were applied. Quantitative analyses GO reveals an atomic percentage of C (57.68 %), O (28.45 %) and Na (13.87 %).
3.3 FTIR Analysis
Fig. 4 shows the FTIR spectrum of the reduced graphene oxide recorded in the range of 4000-400 cm-1. The band appeared at 3321 cm-1 corresponds to O-H stretching vibrations. The peak 2916 cm-1 corresponds to alkanes C-H stretching vibration. The peak 2141cm-1 corresponds to alkynes C – C stretching vibration.
Fig. 3. EDAX image of rGO.
The peak 1695 cm-1 corresponds to C=O stretching and it confirms the presence of alkenes groups. The peak around 1560 cm-1 belongs to out of plane C=C stretching vibration showed the presence of aromatic group. The peak of 1388 cm-1 corresponds to C–OH stretching vibration. The peak of 1215 cm-1 corresponds to C-O stretching vibrations. The peak of 832 cm-1 corresponds to C-O-C stretching vibration. The peak of 533 cm-1 corresponds to NH3torsional oscillation. The intensities of the band associated with oxygen functional groups strongly decreased after reduction of GO by NaBH4. Particularly, the peaks at 1695, 1560, and 1215 cm-1 of GO were significantly reduced in intensity, indicating that the C=O stretching, C=C aromatic, C-O stretching and C-O based functional groups had been removed by sodium borohydride. Hence, NaBH4 is found to be more effective in reducing oxygen functional groups.
Fig. 4.FTIR spectrum of rGO.
3.4 UV-Vis Analysis
The reduction of GO into graphene is characterized by UV−Vis spectroscopy studies as shown in Fig. 5. The UV−vis spectrum of rGO shows an absorption peak at 270 nm which indicates the presence of few layer graphene. For few layer graphene, the absorption peak is expected to be from 230 nm to 270 nm during reduction and also suggesting that GO is reduced.
The electronic conjugation within graphene sheets is restored upon NaBH4 reduction, reflecting increased π-electron concentration due to removal of sp3 carbon atoms and structural ordering, which is consistent with the restoration of sp2 carbon network and possible rearrangement of atoms. In addition, UV-Vis spectra analysis shows a shoulder at 370 nm in rGO, which suggests an absorption peak may be due to Na [26, 27] and an n-π* transition at 350 nm of -C=O bonds [28, 29].
Fig. 5. UV Vis spectrum of rGO
3.5 Cyclic Voltammetry Analysis
3.5.1 Electrochemical properties of rGO sample
Fig. 6 shows CV behavior of bare and rGO modified GCE in presence of 1mM [Fe(CN)6]3-/4- in 1.0 M KCl at a scan rate of 50 mVs-1. CV values for bare and modified rGO are given in table1. Herein, we have noticed a pair of well-defined redox peak ∆Ep values of 73 mV.
Table1. CV current and potential value for Bare and modified rGO.
Deposition of the rGO onto the electrode surface resulted in an irreversible behavior towards [Fe (CN)6]3-/4- voltammetry (∆Ep: 164 mV) as a result of hindrance of electron transfer. Interestingly, the rGO sample redox peaks are increased, due to lattice structures as evidenced by the generation of easily reducible oxygen species which resulted in a better electro catalytic performance.
Notably, the cathodic peak currents showed a significant increase due to enrichment of electron transfer.Electrochemical impedance spectroscopy provides very useful information about the electrochemical process. The behavior of the modified GC electrode is measured by impedance in the frequency region from 100 kHz to 1 Hz and the DC potential 250 mV and AC potential
5 mV in the presence of (1 mM) [Fe (CN)6]3-/4- in 0.1 M KCl. The electron transfer capability of different modified electrodes was investigated by electrochemical impedance analysis and the corresponding results are shown in Fig. 7. The charge transfer resistance (Rct) values were obtained by the Randels equivalent circuit model [Rs(QCPE(Rct(W))] using Zsimpwin software, where Rs is the solution resistance, Rct is the charge transfer resistance, W is the Warburg impedance and QCPE is the constant phase element. The Rct at different stages altered with the successive modification of rGO in [Fe (CN)6]3-/4- as the redox probe.
Fig. 6. Cyclic voltammetry analysis (a) bare GC, (b) rGO modified GCE.
The Rct values of bare and rGO samples were 96.1 and 3919Ω Cm-2 respectively. It shows the electron transfer resistance of the electrode decreased after the modification.
3.5.2 Effect of varying scan rate
Fig. 8 shows the effect of varying scan rates on the cyclic voltammograms performed in 1mM [Fe(CN)6]3-/4- precursor using GO modified electrode in 1.0 M KCl solution was studied over scan rate range of 10mV to 100 mV/s. Oxidation currents were observed to increase with scan rate due to heterogeneous kinetics.
Fig. 7. EIS behavior, (a) bare GC, (b) rGO modified GCE.
Fig. 8. Cyclic voltammetry of rGO modified GCE at different scan rates.
Furthermore, the oxidation and reduction peak currents increased linearly with the square root of scan rate, while the cathodic peak potentials varied linearly with the logarithm of scan rate.
A good linearity was obtained while plotting the current against the scan rate shown in Fig. 9 indicate that the oxidation of [Fe(CN)6]3-/4- was a diffusion controlled process.
Fig. 9.Linear fit plot of current against scan rate.
3.5.3 Sensitivity of the modified electrode using SWV
The sensitivity of the rGO a nanostructure towards the detection of DA & UA was calculated by performing the square wave voltammetry (SWV) at various concentrations. SWV is performed in the potential range -0.1V to 0.8V with 0.1 M phosphate buffer solution with pH 7.0. The DA and UA were injected from 500 nM to1mM into the PBS. In each step of addition of DA & UA, the corresponding current value is noted. It is evident from Fig. 10 (a) and (b), with an increase in the DA & UA concentration. We have witnessed an increase in the peak current.
Fig. 10.SWVs Obtained for various concentrations in the range of 500nM to 1mM at rGO modified GCE in 0.1 PBS pH 7.0 (a) Dopamine (b) Uric Acid.
3.5.4 Individual and simultaneous determination of dopamine (DA) & Uric acid (UA)
A novel modified reduced graphene oxide electrode for the simultaneous determination of dopamine and uric acid has been developed. The modified rGO/GCE film was studied by square wave voltammetry. The effects of various concentration and pulse amplitude were examined to optimize the conditions of square wave voltammetry. The rGO/GCE was well accomplished and determined in the concentration range of 500nM-1mM for Dopamine and Uric Acid. The SWV oxidation peak potential of DA and UA (Fig. 11) were at 0.17V and 0.29V respectively and well defined peak separation of 0.12 V between DA and UA which implies modified electrode successfully prevent the overlap of oxidation potential of two biomolecules. The oxidation peak current of these analytes increased linearly with increased concentration. The linear range for simultaneous determination is 0.5 x 10-6 to 1 x 10-3 M for both DA and UA with limit of detection 0.67 µM and 0.69 µM respectively. The present results are compared to those in literature (Table 2).
The results shows the rGO modified electrodes has better selectivity and sensitivity compared with previous report. Main advantages as for individual and simultaneous detection there is no decreases or increases linear ranges it confirmed the modified electrode shows reasonable biomarker as Parkinson diseases . Thus, rGO with unique structural properties are promising candidate for biosensor and bioelectronics.