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Document Type : Original Research Article


Directorate of Forensic Science Laboratories, Government of Maharashtra, India


The forensic DNA fingerprinting technique play a vital role during the investigation of rape and murder cases and gives strong scientific evidence for linking the suspect with the scene of crime and victim. We present a case along with an analysis technique that will bring about the importance of forensic biology and DNA technique of how forensic science is helpful to give conclusions to the rape and murder case. In forensic biology, blood typing was an important forensic tool but blood typing was not a very discriminating technique. The blood group of victims and suspects may be similar in most cases so the use of DNA profiling has increased. DNA can be extracted from bloodstains and semen proves an act of rape and murder.

Graphical Abstract

Importance of forensic techniques in the investigation of rape (sodomy) and murder


* Link of suspect with the scene of the crime and proves an act of sodomy.

* Sodomy and murder by the same suspect prove the act of serial killing.

* DNA fingerprinting provides a useful tool for the identification of the suspect


Main Subjects

[1]Dr.K.S.Narayan Reddy the essentials of forensic medicine and toxicology; 34th edition. Page no. 399
[2] T. Murakami, “Application of the Scanning Electron Microscope to the Study of the Fine Distribution   of the Blood Vessels.”ArchHistolJpn. 32 (1971): 445
[3] Nopanitaya, Waykin, JG Aghajanian and LD Gray.“An Improved Plastic Mixture for Corrosion Casting of the Gastrointestinal Microvascular System.”Scan Electron Microsc. (1979): 751-755.
 [4] S. Nath, HK. Pratihari (2018) Gang Rape and Murder – A Case Report. J Forensic Sci& Criminal Inves 10(1): 1-4.
 [5]Fisher, AJ. Barry (2004) Techniques of crime scene investigation, 7th Edition, CRC Press LLC, Boca Raton.
 [6] Becker, F. Ronald (2009) Criminal Investigation, 3rd Edition, Jones and Bartlett Publishers, Sudbury, Massachusetts.
[7] A.J Jaffreys, JKY Brookfield, R. Semenoff Positive identification of an immigration test
case using human DNA fingerprints. Nature 1985; 317: 818-9.
 [8]S. G. Pawar, D. Y. Kudekar, B. P. More, K. V. Kulkarni; Role of Short Tandem Repeats from trace DNA in Forensic Casework, Journal of Emerging Technologies and Innovative Research(JETIR)7, 11(2020).
 [9]D. Y. Kudekar, V. B. Mahajan, B. P.More, J. R. Kawade; Blood of Accused – Vital Scientific Evidence in Crime of Rape, Journal of Forensic Investigation8, 2(2020).
[10]V. B. Mahajan, S. K. Gajare, D. Y. Kudekar, B. P. More, A. K. Rangari, N. R. Gosavi, K. V. Kulkarni; Importance of DNA Profiling and Botanical Examination to fix the offence and offence place, International Journal of Current Advanced Research, 9, 1 (2020).
[11]V. B. Mahajan, D. Y. Kudekar, B. P. More, K. V. Kulkarni; Life Imprisonment for Raping to a Toddler-success of DNA Profiling, International Journal of Innovations in Biological and Chemical Sciences13 (2020).
[12]MG. Brevnov, J. Mundt, LM. Calandro, MR Furtado, et al. (2009) Developmental validation of the Prep Filer Forensic DNA Ex­traction Kit for extraction of genomic DNA from biological samples. J Forensic Sci 54(3):599-607.
[13]ThermoFisher Scientific, PrepFiler Express and AutoMate Express DNA extraction systems Manual.