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Document Type : Original Research Article


Department Insect Population Toxicology, Central Agricultural Pesticides Laboratory, Agriculture Research Center, Dokki, Giza, Egypt


Plant essential oils (EOs) were tested against S. littoralis larvae. Responses varied according to EOs species and all EOs were proved to be toxic to the larvae. However, the highest mortality was observed in the oils of orange, sesamum, and camphor. The slope values indicated that the insect population was relatively heterogeneous in their susceptibility toward tested EOs. Our results showed LC50 values, the range of toxicity was in the decreasing order of orange˃ sesamum˃ camphor˃ pepper˃ pumpkin against both S. littoralis 2nd and 4th instars. The influence of LC25 values of biological parameters manifested in decreased the adult emergence and prolongation of the larval and pupal period. The percentage of larvae that survived and succeeded to pupate decreased the result of treatment by LC25 values. Longevity, fecundity, fertility and natality were monitored in the surviving adults. The most significant effect of EOs was determined with respect to natality. Compared to the control, all the tested EOs caused a significant reduction in natality. While 1210.2 viable larvae were obtained in the control, the number of viable larvae obtained from those treated was lower, ranging from 35.8 % (for orange oil) up to 81.9 % (for pumpkin oil). Based on the presented results, EOs, orange, sesamum, and camphor. Generally, sublethal effects of EOs exhibit significant effects on the population dynamics of S. littolaris where, reduction of adult natality by more than 75 % in this pest.

Graphical Abstract

Sublethal Effects of Some Essential Oils on the Developmental and Reproduction of the Spodoptera littoralis (Boisduval)


Main Subjects

[1] S.M. Ismail, F.A. Abdel-Galil, Sh.H. Sameh, U.M. Abu El-Ghiet, Influence of some insecticides on the incidence of common Lepidopterous insect pests in cotton field. Egypt. Acad. J. Biolog. Sci., 12 (2020) 23-30.                
[2] C. Regnault-Roger, C. Vincent, J.T. Arnason, Essential oil in insect control: low-risk products in a high-stakes world. Annual Rev. Entomol., 57 (2012)405-424.   
[3] E.V.R. Campos, P.L.F. Proenca, J.L. Oliveira, M. Bakshi, P.C. Abhilash, L.F. Fraceto, Use of botanical insecticides for sustainable agriculture: futureperspectives. Ecol. Indic., 105 (2019) 483-496. 
[4] S.M. Ismail, M. Morshedy, The interaction of some plant essential oils on the toxicity and biochemical effect of phenothrin on Spodoptera littoralis larvae. Alex. Sci. Exchenge J., 28 (2007) 109-114.
[5] K. Opender, W. Suresh, G.S. Dhaliwal, Essential oils as green pesticides: potential and constraints. Biopestic. Int., 4 (2008) 63–84.
[6] L.M.L. Nollet, H.S. Rathore, Green pesticides Handbook Essential Oil for Pest Control. CRC Press, (2017).         
[7] S.M. Ismail, N. Shaker, Efficacy of some essential oils against immature stages of Spodoptera littoralis. Alex. J. Agric. Res. 59 (2014) 97-103. 
[8] S.M. Eridiane, R.D.A.F. Lêda, C.Z. José, F.H. Fernanda, H.F.P. Lucas, Insecticidal activity of Vanillosmopsis arborea essential oil and of its major constituent α-bisabolol against Callosobruchus maculatus (Coleoptera: Chrysomelidae). Scientific Repots, 9 (2019) 3723-3731.
[9] B. Daniel, J. Chuleui, Insecticidal toxicities of three main constituents derived from Trachyspermum ammi (L.) sprague ex turrill fruits against the small hive beetles. Molecules, 25 (2020) 2-12.
[10] L.A. Hummelbrunner, M.B. Isman, Acute, sublethal, antifeedant, and    synergistic effects of monoterpenoid essential oil compounds on the tobacco cutworm, Spodoptera litura (Lep., Noctuidae). J. Agric. Food Chem. 49 (2001) 715-720.
[11] D.P. Papachristos, D.C. Stamopoulos, Sublethal effects of three essential oils on the development, longevity, and fecundity of Acanthoscelides obtectus (Say) (Coleoptera: Bruchidae). Hellenic Plant Prot. J. 2 (2009) 91-99.
[12] R. Pavela, Natural products as allelochemicals in pest management. In: Dubey, N. (ed.). Natural products in plant pest management. CAB International, (2010) pp. 134-148.
[13] W.S. Abbott, A method for computing the effectiveness of an insecticide. J.  Econ. Entomol.,18 (1925) 265-267.
[14] D.J. Finney, Probit analysis.3rd ed., Cambridge Univ. Press, London: (1971) 318p. 
[15] K. Zarrad, I. Chaieb, S. Souguir, W. Tayeb, I. Chraief, A. Laarif, M. Hammami, R. Haouala, Bio-insecticidal potential of essential oils of two Citrus species against two Greenhouse pests Tuta absoluta Meyrick and Spodoptora littoralis Boisduval. Microbiol, Hyg. Alim., 25 (2013) 84-88.
[16] N. Dhen, O. Majdoub, S. Souguir, W. Tayeb, A. Laarif, I. Chaieb, Chemical composition and fumigant toxicity of Artemisia absinthium essential oil against Rhyzopertha dominica and Spodoptera littoralis. Tun. J. Plant Prot., 9 (2014) 57-65.
 [17] S. Rguez, K. Msaada, M. Daami-Remadi, I. Chaieb, R.I. Bettaieb, M. Hammami, A. Laarif, I. Hamrouni-Sellami, Chemical composition andbiological activities of essential oils of Salvia officinalis aerial parts as affected by diurnal variations. Plant Biosystems, (2018) 1-9.
[18] M.P. Zhao, Q.Z. Liu, Q. Liu, Z.L. Liu, Identification of larvicidal constituents of the essential oil of Echinops grijsii roots against the three species of mosquitoes. Molecules, 22 (2017) 205-219.
[19] Ch. Kamaraj, R.G. Pachiyappan, S. Gandhi, C.H. Min, G. Rajakamura, Novel and environmental friendly approach: impact of neem (Azadirachta Indica) Gum Nano Formulation (NGNF) on Helicoverpa armigera (Hub.) and Spodoptera litura (Fab.). Inter. J. Biol. Macromolecules, 107 (2018) 59-69. 
[20] R. Rizzo, G.L. Verde, M. Sinacori, F. Maggi, L. Cappellacci, R. Petrelli, S. Vittori, M.R. Morshedloo, G.B.Y. Fofie, G. Benelli, Developing green insecticides to manage olive fruit flies? Ingestion toxicity of fouressential oils in protein baits on Bactrocera oleae. Ind. Crop. Prod., 143 (2020) 111884-111891.
[21] R. Singh, O. Koul, P.J. Rup, Effect of some essential oil compounds on the oviposition and feeding behavior of the Asian armyworm, Spodoptera litura F. (Lepidoptera: Noctuidae). Biopest. Internat., 6 (2010) 52-66.
[22] M. Alkan, A. Gokce, K. Kara, Contact toxicity of six plant extracts to different larvae stages of Colorado potato beetle (Leptinotarsa decemlineata SAY (Col: Chrysomelidae). J. Agricul. Sci., 23 (2017) 309-316.
[23] T. Sorachat, Y. Thitaree, P. Anchulee, R. Atcharee, K. Nutchaya, B. Vasakorn, P. Wanchai, Synthesis of thymyl esters and their insecticidal activity againstSpodoptera litura (Lepidoptera: Noctuidae). Pest Management Sci., 76 (2020) 928-935.