TY - JOUR ID - 104776 TI - Synthesis of liponucleotides using bacterial phospholipase D JO - Progress in Chemical and Biochemical Research JA - PCBR LA - en SN - 2676-7090 AU - Birichevskaya, Larisa AU - Vinter, Margarita AU - Litvinko, Natalia AU - Zinchenko, Anatoli AD - Institute of Microbiology, National Academy of Sciences of Belarus, Minsk-220141, Belarus AD - Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus, Minsk-220141, Belarus Y1 - 2020 PY - 2020 VL - 3 IS - 1 SP - 64 EP - 73 KW - Phospholipid prodrugs KW - Streptomyces netropsis KW - Enzymatic transphosphatidylation KW - Modified nucleosides KW - Phosphatidylclofarabine DO - 10.33945/SAMI/PCBR.2020.1.8 N2 - A series of conjugates of pharmacologically promising modified nucleosides with phospholipid was synthesized. Some liponucleotides were originally produced. Soybean lecithin served as the donor of phosphatidyl residue. Phospholipase D from strain Streptomyces netropsis BIM B-428D was engaged as biocatalyst in transphosphatidylation reaction. The yield of liponucleotide synthesis reaction varied in the range 44–95 mol% depending on the acceptor of phosphatidyl residue. 65 mg (about 68 µmoles) of pure phosphatidylclofarabine was recovered by chromatography on silica gel column, resulting in 68 mol% yield calculated from the amount of modified nucleoside supplied into the reaction mixture. UR - https://www.pcbiochemres.com/article_104776.html L1 - https://www.pcbiochemres.com/article_104776_235ffd92bba60416c14a2f90d538e8ce.pdf ER -