Document Type: Original Research Article

Authors

1 Department of Chemistry, KSKV Kachchh University, Bhuj, Kachchh- 370001

2 Department of Chemistry, Sardar Patel University, Vallabh Vidynagar-388 120 (Gujarat), India

10.33945/SAMI/PCBR.2019.2.1319

Abstract

The current study was carried out on the extraction, isolation, and identification of phytochemical constituents existing in the Abutilon pannosum and Grewia tenax leaves extract collected from Kachchh region of Gujarat state, India. The main aim of this study was to expose important polar phytochemicals in locally available medicinal plants using LC-Q-TOF-MS analytical techniques. The main analytical tool is liquid chromatography quadrupole time of flight mass spectrometry (LC-Q-TOF-MS) which is one of the most sophisticated and sensitive instruments that gives qualitative as well as quantitative information accurately with respect to constituents present in the sample. The continuous extraction of plant leaves was commenced by Soxhlet extractor utilizing methanol as a solvent. The crude methanolic extracts were inserted in LC-Q-TOF-MS for identification and isolation of useful phytochemicals. The results of phytochemical analysis displayed that there were significant phytochemicals found in methanol extracts of A. pannosum leaves (APL) such as alkaloids, photoproteins, antibioticand in G. tenax leaves (GTL) like alkaloids, photoproteins, glycoside, terpenoids, fatty amides, steroids, fatty alcohols, saponins, flavones, flavonols, steroid etc. Hence, methanolic extract of GTL gives good medicinal activity as compared to the APL.

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[1]    Iqbal E., Salim K. A., Lim L. B. L., J. King Saud Univ.Sci., 2015,27:224

[2]    Nostro A., Germanò M. P., D'angelo V., Marino A., Cannatelli M. A.,Lett.ApplMicrobiol, 2000,30:379

[3]    Shevchenko A.A., Chernushevich I.V., Ens W., Standing K.G., Thomson B., Wilm M, Mann M.,Rapid Commun Mass Spectrom.,1997, 11:1015

[4]    Abedin S.,Pak. J. Bot., 1980,12:43

[5]    Sammia Y., Studies on bioactive natural products of selected species of family Malvaceae,Ph. D. Thesis, Department of Chemistry GC University, Lahore, 2008

[6]    Anup A.,Bioscience Discovery, 2012,3:317-320

[7]    Habiba U., Anwar M., Hussain I., Rais M.,J. AnimalPlantSci., 2015,25:650

[8]    Aboagarib E., Yang R., Hua X., Siddeeg A., J. Food Nutrit. Res.,2014,2:187

[9]    Tan H. Y. et al., Evidence-based Complement. Altern. Med., 2016

[10]Sharma N., Patni V., Asian J. Pharm.Clin. Res., 2012, 5:28

[11]Khemiss F., Ghoul-Mazgar S., Moshtaghie A., Saidane D., J. Ethnopharm. 2006,103:90

[12]Gebauer J., Patzelt A., Hammer K., Buerkert A., Genet. Resour. Crop Evol., 2007, 54:1153

[13]DaffallaH. M., ElsheikhA. M., AliH. A., and KhalfalaM. M., Environ. Exp. Biol., 2016, 14:75

[14]El Ghazali G.E.B., El Tohami M.S., El Egami A.A.B., Abdalla W.S., Mohammed M.G.,Omdurman Islamic University Printing and Publishing House, Omdurman, 1997

[15]Mishra D. N., Thorat A. K., Int. J. ChemTech Res., 2017, 10:723

[16]Lebreton J., Synthesis (Stuttg), 2002, 9192

[17]Doganci S. et al., Eur. Rev. Med. Pharmacol. Sci., 2016, 1

[18]Aadesariya M. K., Ram V. R., Dave P. N., Int. J. Adv. Res. Chem. Sci., 2017, 4(10); 1

[19]Abdelhady M. I. S., Motaal A. A., Brazilian J. Pharmacogn., 2016, 26:763

[20]A. Maroyi,The Scientific World Journal, 2018, 2018:1

[21]Qingwei W. et al., Molecules, 2012, 17:12330

[22]De Sousa E. A., Da Silva A. A. C. A., Cavalheiro A. J., Lago J. H. G., Chaves M. H., J. Braz. Chem. Soc., 2014, 25:704

[23]Varsha. G. J., Int. J. Environ. Sci. Nat. Resour., 2017, 4:1

[24]Tapondjou L.A., Jenett-Siems K., Bottger S., Melzig M.F.,Phytochemistry, 2013, 95:341

[25]Hastings J.W., J. Mol. Evol.,1983, 19:309

[26]Akpabio U. D., Udo U. E., Akpakpan A. E., Asian J. Plant Sci. Res., 2012, 2:607

[27]Symersky J., Osowski D., Walters D. E., Mueller D. M., Proc. Natl. Acad. Sci., 2012, 109:13961

[28]Plazonić A., Molecules,2009, 14:2466

[29]Pattanaik B., Lindberg P., Life, 2015, 5:269

[30]Linn C., Lakshmi V., Aa M., Kumar R., Herb. Med., 2017, 1–5

[31]Aadesariya M. K., Ram V. R., Dave P. N., Int. J. Adv. Res. Chem. Sci., 2017, 4:1

[32]Asif M., Khodadadi E., J. Paramed. Sci., 2013, 4:119

[33]Geetha T. S., Geetha N., Int. J. PharmTech Res., 2014, 6:521

[34]Krishnaiah T., Bono A., Sarbatly R., J. Med. Plants Res., 2009, 3:67

[35]Murugan M., Mohan V. R., J. Med. Plants Stud., 2014, 2:51