Document Type : Original Research Article


1 Department of Pure and Industrial Chemistry, Nnamdi Azikiwe University, Awka, Nigeria.

2 Department of Chemistry, Federal University of Technology, Owerri, Nigeria.

3 Directorate of Chemical Evaluation and Regulation, National Agency for Food, Drug Administration and Control, Lagos, Nigeria, Nigeria.


 Phytochemical and antimicrobial analysis of Moringa oleifera seeds were carried using standard laboratory procedures. The phytochemical content showed the presence of alkaloid, flavonoid, saponin, and steroids respectively as samples contained 5.85g alkaloid, 5.97g flavonoid, and 1.46g saponin. Anti-microbial screening with certain selected human pathogens and the zones of inhibition obtained showed that alkaloids were 3mm, 3mm, 2mm, 4mm, & 5mm, flavonoids were 6mm, 5mm, 4mm, 2mm and 7mm and saponins were 4mm, 3mm, 3mm, 6mm and 5mm for Escherichia coli, Pseudomonas aureginosa, Klebsiella, Staphylococcus aureus and Streptocuccus aureus. Minimum inhibitory concentration (MIC) were 6.5mg/cm3, 12.5mg/cm3, 12.5mg/cm3, 6.5mg/cm3 and 12.5mg/cm3 for Escherichia coli, Pseudomonas aureginosa, Klebsiella, Staphylococcus aureus and Streptocuccus aureus respectively. This finding shows that the application of the seed extract of Moringa oleifera for potential therapeutic use and medicinal drugs in treatment of pneumonia, urinary tract infection and a host of other diseases in humans.

Graphical Abstract

Phytochemical and Anti-Microbial Analysis of Metabolites in seeds of Moringa oleifera grown in Nigeria


  • Moringa oleiferaexist majorly in the tropics
  • It has economic use in water treatment and production of perfumes and hair creams
  • It has nutritional uses in the formulation of food supplements and animal feeds
  • Phytochemical and antimicrobial assessment showed it has therapeutic use in treatment of diseases


Main Subjects

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