Document Type: Original Research Article

Authors

1 Botany Department, Faculty of Science, Mansoura University, ET-35516, Mansoura, Egypt.

2 Physics Department, Faculty of Science, Mansoura University, ET-35516, Mansoura, Egypt.

3 Unit of Genetic Engineering and Biotechnology, Faculty of Science, Mansoura University, ET-35516, Mansoura, Egypt.

Abstract

Crataegus sinaica Boiss is a hawthorn plant that was found as a hybrid of two species, C. azarolus and C. monogyna, which grows vastly in the mountains of the Protectorate of St. Catherine, South Sinai, Egypt. The fruits of the plant are rich in primary and secondary metabolites, for instance reducing, total sugars, flavonoids, and phenols as demonstrated by the phytochemical analysis. The aqueous extract of the fruits of the plant was used to prepare the silver nanoparticles by green method, in which the reducing and total sugars facilitate the preparation step as they act as reducing and stabilizing agents. The nanoparticles of the plant were efficiently synthesized through mixing Crataegus sinaica fruits aqueous extract with silver nitrate solution at room temperature following the predetermined procedures for nanoparticle preparation. The prepared nanoparticles were identified by means of spectroscopic and analytical measurements i.e. UV-vis, IR, TEM, and zeta sizer-zeta analyzer. The extract of the fruits of the plant and its silver nanoparticles were assessed as antimicrobial and antioxidant agents, in which the nanoparticle solution displayed the more potent activities against the diverse microbial species and potent antioxidant agent than the aqueous extract.

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Main Subjects

[1] S.U. Khan, T.A. Saleh, A. Wahab, M.H.U. Khan, D. Khan, W.U. Khan, A. Rahim, S. Kamal, F.U. Khan, S. Fahad, Nanosilver: new ageless and versatile biomedical therapeutic scaffold, Int. J. Nanomedicine. 13 (2018) 733.

[2] A. Taraszkiewicz, G. Fila, M. Grinholc, J. Nakonieczna, Innovative strategies to overcome biofilm resistance, Biomed Res. Int. 2013 (2012).

[3] G. Franci, A. Falanga, S. Galdiero, L. Palomba, M. Rai, G. Morelli, M. Galdiero, Silver nanoparticles as potential antibacterial agents, Molecules. 20 (2015) 8856–8874.

[4] Z. Wang, Q. Li, Y. Chen, B. Cui, Y. Li, F. Besenbacher, M. Dong, The ambipolar transport behavior of WSe 2 transistors and its analogue circuits, NPG Asia Mater. 10 (2018) 703–712.

[5] A.P. Reverberi, N.T. Kuznetsov, V.P. Meshalkin, M. Salerno, B. Fabiano, Systematical analysis of chemical methods in metal nanoparticles synthesis, Theor. Found. Chem. Eng. 50 (2016) 59–66.

[6] N. Skandalis, A. Dimopoulou, A. Georgopoulou, N. Gallios, D. Papadopoulos, D. Tsipas, I. Theologidis, N. Michailidis, M. Chatzinikolaidou, The effect of silver nanoparticles size, produced using plant extract from Arbutus unedo, on their antibacterial efficacy, Nanomaterials. 7 (2017) 178.

[7] A.T. Refaat, A.A. Shahat, N.A. Ehsan, N. Yassin, F. Hammouda, E.A. Tabl, S.I. Ismail, Phytochemical and biological activities of Crataegus sinaica growing in Egypt, Asian Pac. J. Trop. Med. 3 (2010) 257–261.

[8] S. Ahmad, S. Munir, N. Zeb, A. Ullah, B. Khan, J. Ali, M. Bilal, M. Omer, M. Alamzeb, S.M. Salman, Green nanotechnology: a review on green synthesis of silver nanoparticles—an ecofriendly approach, Int. J. Nanomedicine. 14 (2019) 5087.

[9] A. Moustafa, M. Zaghloul, S. Mansour, M. Alotaibi, Conservation Strategy for protecting Crataegus x sinaica against climate change and anthropologic activities in South Sinai Mountains, Egypt, Catrina Int. J. Environ. Sci. 18 (2019) 1–6.

[10]     L. Boulos, Flora of egypt, Al Hadara Publishing Cairo, 2005.

[11]     K. Wolfe, X. Wu, R.H. Liu, Antioxidant activity of apple peels, J. Agric. Food Chem. 51 (2003) 609–614.

[12]     J. Zhishen, T. Mengcheng, W. Jianming, Research on antioxidant activity of flavonoids from natural materials, Food Chem. 64 (1999) 555–559.

[13]     B. Thayumanavan, S. Sadasivam, Physicohemical basis for the preferential uses of certain rice varieties, Plant Foods Hum. Nutr. 34 (1984) 253–259.

[14]     D.D. Kitts, A.N. Wijewickreme, C. Hu, Antioxidant properties of a North American ginseng extract, Mol. Cell. Biochem. 203 (2000) 1–10.

[15]     I. Parejo, C. Codina, C. Petrakis, P. Kefalas, Evaluation of scavenging activity assessed by Co (II)/EDTA-induced luminol chemiluminescence and DPPH·(2, 2-diphenyl-1-picrylhydrazyl) free radical assay, J. Pharmacol. Toxicol. Methods. 44 (2000) 507–512.

[16]     S. Pirtarighat, M. Ghannadnia, S. Baghshahi, Green synthesis of silver nanoparticles using the plant extract of Salvia spinosa grown in vitro and their antibacterial activity assessment, J. Nanostructure Chem. 9 (2019) 1–9.

[17]     Z.R. Zad, S.S.H. Davarani, A. Taheri, Y. Bide, A yolk shell Fe3O4@PA-Ni@Pd/Chitosan nanocomposite -modified carbon ionic liquid electrode as a new sensor for the sensitive determination of fluconazole in pharmaceutical preparations and biological fluids, Journal of Molecular Liquids, 253(2018) 233-40.

[18]     S. Sardari, G. Amin, R.G. Micetich, M. Daneshtalab, Phytopharmaceuticals. Part 1. Antifungal activity of selected Iranian and Canadian plants, Pharm. Biol. 36 (1998) 180–188.

[19]     N. Durán, M. Durán, M.B. De Jesus, A.B. Seabra, W.J. Fávaro, G. Nakazato, Silver nanoparticles: A new view on mechanistic aspects on antimicrobial activity, Nanomedicine Nanotechnology, Biol. Med. 12 (2016) 789–799.

[20]     L. Salvioni, E. Galbiati, V. Collico, G. Alessio, S. Avvakumova, F. Corsi, P. Tortora, D. Prosperi, M. Colombo, Negatively charged silver nanoparticles with potent antibacterial activity and reduced toxicity for pharmaceutical preparations, Int. J. Nanomedicine. 12 (2017) 2517.

[21]     O.A. El-Shahaby, M. El-Zayat, A. El-Fattah, M.M. El-Hefny, Evaluation of the biological activity of Capparis spinosa var. aegyptiaca essential oils and fatty constituents as Anticipated Antioxidant and Antimicrobial Agents, Prog. Chem. Biochem. Res. 2 (2019) 211–221.

[22]     O. El-Shahaby, M. El-Zayat, R. Rabei, H.S. Aldesuquy, Phytochemical constituents, antioxidant activity and antimicrobial potential of Pulicaria incisa (lam.) DC as a folk medicinal plant, Prog. Chem. Biochem. Res. 2 (2019) 222–227.

[23]     D.M. Maestri, V. Nepote, A.L. Lamarque, J.A. Zygadlo, Natural products as antioxidants, Phytochem. Adv. Res. 37 (2006) 105–135.

[24]     R. Jalilian, M. Shahmari, A. Taheri, K. Gholami, Ultrasonic-assisted micro solid phase extraction of arsenic on a new ion-imprinted polymer synthesized from chitosan-stabilized pickering emulsion in water, rice and vegetable samples, Ultrason Sonochem, 61(2020) 104802.

[25]     E.M. Egorova, A.A. Revina, Synthesis of metallic nanoparticles in reverse micelles in the presence of quercetin, Colloids Surfaces A Physicochem. Eng. Asp. 168 (2000) 87–96.

[26]     S. Ghosh, S. Patil, M. Ahire, R. Kitture, S. Kale, K. Pardesi, S.S. Cameotra, J. Bellare, D.D. Dhavale, A. Jabgunde, Synthesis of silver nanoparticles using Dioscorea bulbifera tuber extract and evaluation of its synergistic potential in combination with antimicrobial agents, Int. J. Nanomedicine. 7 (2012) 483.

[27]     K.I. Batarseh, Anomaly and correlation of killing in the therapeutic properties of silver (I) chelation with glutamic and tartaric acids, J. Antimicrob. Chemother. 54 (2004) 546–548.

[28]     V.K. Sharma, R.A. Yngard, Y. Lin, Silver nanoparticles: green synthesis and their antimicrobial activities, Adv. Colloid Interface Sci. 145 (2009) 83–96.

[29]     M. Tammer, G. SInfrared and Raman characteristic group frequencies: tables and charts, (2004).

[30]     O. El-Shahaby, M. El-Zayat, E. Salih, I.M. El-Sherbiny, F.M. Reicha, Evaluation of antimicrobial activity of water infusion plant-mediated silver nanoparticles, J Nanomed Nanotechol. 4 (2013) 2.

 

 

 

 

 

HOW TO CITE THIS ARTICLE

O.A. EL-Shahaby, F.M. Reicha, M. M. Nabil Aboushadi, M.M. El-Zayat, Green Synthesis and Biological Assessments of Silver Nanoparticles Using the Plant Extract of Crataegus sinaica Boiss. Fruits, Prog. Chem. Biochem. Res. 2020, 3(2),105-113.

DOI: 10.33945/SAMI/PCBR.2020.2.3