@article { author = {Birichevskaya, Larisa and Vinter, Margarita and Litvinko, Natalia and Zinchenko, Anatoli}, title = {Synthesis of liponucleotides using bacterial phospholipase D}, journal = {Progress in Chemical and Biochemical Research}, volume = {3}, number = {1}, pages = {64-73}, year = {2020}, publisher = {Sami Publishing Company}, issn = {2676-7090}, eissn = {2645-6133}, doi = {10.33945/SAMI/PCBR.2020.1.8}, abstract = {A series of conjugates of pharmacologically promising modified nucleosides with phospholipid was synthesized. Some liponucleotides were originally produced. Soybean lecithin served as the donor of phosphatidyl residue. Phospholipase D from strain Streptomyces netropsis BIM B-428D was engaged as biocatalyst in transphosphatidylation reaction. The yield of liponucleotide synthesis reaction varied in the range 44–95 mol% depending on the acceptor of phosphatidyl residue. 65 mg (about 68 µmoles) of pure phosphatidylclofarabine was recovered by chromatography on silica gel column, resulting in 68 mol% yield calculated from the amount of modified nucleoside supplied into the reaction mixture.}, keywords = {Phospholipid prodrugs,Streptomyces netropsis,Enzymatic transphosphatidylation,Modified nucleosides,Phosphatidylclofarabine}, url = {https://www.pcbiochemres.com/article_104776.html}, eprint = {https://www.pcbiochemres.com/article_104776_235ffd92bba60416c14a2f90d538e8ce.pdf} }